Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages43-49
Number of pages7
Volume1150
DOIs
Publication statusPublished - 2009
Event3rd International Meeting on Frontiers in Physics, IMFP 2009 - Kuala Lumpur, Malaysia
Duration: 2009 Jan 122009 Jan 16

Other

Other3rd International Meeting on Frontiers in Physics, IMFP 2009
CountryMalaysia
CityKuala Lumpur
Period09/1/1209/1/16

Fingerprint

microscopy
fluorescence
photons
pulses
excitation
lasers
color
crosstalk
phase modulation
cells

Keywords

  • Biomedical imaging
  • Fluorescence
  • Multiphoton processes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Isobe, K., Suda, A., Hashimoto, H., Kannari, F., Kawano, H., Mizuno, H., ... Midorikawa, K. (2009). Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses. In AIP Conference Proceedings (Vol. 1150, pp. 43-49) https://doi.org/10.1063/1.3192289

Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses. / Isobe, Keisuke; Suda, Akira; Hashimoto, Hiroshi; Kannari, Fumihiko; Kawano, Hiroyuki; Mizuno, Hideaki; Miyawaki, Atsushi; Midorikawa, Katsumi.

AIP Conference Proceedings. Vol. 1150 2009. p. 43-49.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Isobe, K, Suda, A, Hashimoto, H, Kannari, F, Kawano, H, Mizuno, H, Miyawaki, A & Midorikawa, K 2009, Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses. in AIP Conference Proceedings. vol. 1150, pp. 43-49, 3rd International Meeting on Frontiers in Physics, IMFP 2009, Kuala Lumpur, Malaysia, 09/1/12. https://doi.org/10.1063/1.3192289
Isobe K, Suda A, Hashimoto H, Kannari F, Kawano H, Mizuno H et al. Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses. In AIP Conference Proceedings. Vol. 1150. 2009. p. 43-49 https://doi.org/10.1063/1.3192289
Isobe, Keisuke ; Suda, Akira ; Hashimoto, Hiroshi ; Kannari, Fumihiko ; Kawano, Hiroyuki ; Mizuno, Hideaki ; Miyawaki, Atsushi ; Midorikawa, Katsumi. / Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses. AIP Conference Proceedings. Vol. 1150 2009. pp. 43-49
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