Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.

Original languageEnglish
Title of host publicationFrontiers in Physics - 3rd International Meeting, IMFP 2009
Pages43-49
Number of pages7
DOIs
Publication statusPublished - 2009 Nov 25
Event3rd International Meeting on Frontiers in Physics, IMFP 2009 - Kuala Lumpur, Malaysia
Duration: 2009 Jan 122009 Jan 16

Publication series

NameAIP Conference Proceedings
Volume1150
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other3rd International Meeting on Frontiers in Physics, IMFP 2009
CountryMalaysia
CityKuala Lumpur
Period09/1/1209/1/16

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Keywords

  • Biomedical imaging
  • Fluorescence
  • Multiphoton processes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Isobe, K., Suda, A., Hashimoto, H., Kannari, F., Kawano, H., Mizuno, H., Miyawaki, A., & Midorikawa, K. (2009). Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses. In Frontiers in Physics - 3rd International Meeting, IMFP 2009 (pp. 43-49). (AIP Conference Proceedings; Vol. 1150). https://doi.org/10.1063/1.3192289