Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.

    Original languageEnglish
    Title of host publicationFrontiers in Physics - 3rd International Meeting, IMFP 2009
    Pages43-49
    Number of pages7
    DOIs
    Publication statusPublished - 2009 Nov 25
    Event3rd International Meeting on Frontiers in Physics, IMFP 2009 - Kuala Lumpur, Malaysia
    Duration: 2009 Jan 122009 Jan 16

    Publication series

    NameAIP Conference Proceedings
    Volume1150
    ISSN (Print)0094-243X
    ISSN (Electronic)1551-7616

    Other

    Other3rd International Meeting on Frontiers in Physics, IMFP 2009
    CountryMalaysia
    CityKuala Lumpur
    Period09/1/1209/1/16

    Keywords

    • Biomedical imaging
    • Fluorescence
    • Multiphoton processes

    ASJC Scopus subject areas

    • Physics and Astronomy(all)

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  • Cite this

    Isobe, K., Suda, A., Hashimoto, H., Kannari, F., Kawano, H., Mizuno, H., Miyawaki, A., & Midorikawa, K. (2009). Multifarious two-photon fluorescence microscopy employing ultrabroadband femtosecond laser pulses. In Frontiers in Physics - 3rd International Meeting, IMFP 2009 (pp. 43-49). (AIP Conference Proceedings; Vol. 1150). https://doi.org/10.1063/1.3192289