Multilaminar/vesicular bodies accumulating glycoconjugates in primary spermatocytes of cricket, Gryllus bimaculatus

Atsushi C. Suzuki, Kenji Nishimura

Research output: Contribution to journalArticle

Abstract

Lectin cytochemistry was carried out on thin sections of 6th-instar cricket testis using two GalNAc-specific lectins, Dolichos biflorus agglutinin (DBA) and Helix pomatia agglutinin (HPA), and the binding sites in primary spermatocytes were surveyed. Gold particles showing DBA-binding are observed specifically in dense-body clusters. These bodies are about 100-300 nm in diameter and exhibit multilaminar or multivesicular structure. HPA can bind to the dense-body clusters and another kind of larger multivesicular structures. These bodies seem to contain some heterogeneous substances, and sometimes show an autophagosome-like structure. The ultrastructures of these organelles surveyed in conventional Epon sections confirmed the structures of these multilaminar/vesicular bodies in cricket spermatocytes, which may play certain roles in intracellular circulation and degradation of glycoconjugates.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalZoological Science
Volume15
Issue number1
DOIs
Publication statusPublished - 1998 Feb

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ASJC Scopus subject areas

  • Animal Science and Zoology

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