Multimeric bivalent immunogens from recombinant tetanus toxin HC fragment, synthetic hexasaccharides, and a glycopeptide adjuvant

Aileen F.G. Bongat, Rina Saksena, Roberto Adamo, Yukari Fujimoto, Zenyu Shiokawa, Dwight C. Peterson, Koichi Fukase, Willie F. Vann, Pavol Kovac

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Using recombinant tetanus toxin HC fragment (rTT-HC) as carrier, we prepared multimeric bivalent immunogens featuring the synthetic hexasaccharide fragment of O-PS of Vibrio cholerae O:1, serotype Ogawa, in combination with either the synthetic hexasaccharide fragment of O-PS of Vibrio cholerae O:1, serotype Inaba, or a synthetic disaccharide tetrapeptide peptidoglycan fragment as adjuvant. The conjugation reaction was effected by squaric acid chemistry and monitored in virtually real time by SELDI-TOF MS. In this way, we could prepare well-defined immunogens with predictable carbohydrate-carrier ratio, whose molecular mass and the amount of each saccharide attached could be independently determined. The ability to prepare such neoglycoconjugates opens unprecedented possibilities for preparation of conjugate vaccines for bacterial diseases from synthetic carbohydrates.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalGlycoconjugate Journal
Volume27
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Keywords

  • Adjuvant
  • Conjugate vaccine
  • Squaric acid
  • Tetanus toxin C fragment
  • Vibrio cholerae

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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