Multiple deletions in mitochondrial DNA at direct repeats of non-D-loop regions in cases of familial mitochondrial myopathy

Michisuke Yuzaki, Norio Ohkoshi, Ichiro Kanazawa, Yasuo Kagawa, Shigeo Ohta

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Muscle mitochondrial DNAs from two brothers with mitochondrial myopathy associated with peripheral neuropathy had multiple deletions, most of which started in non-D-loop regions, unlike in an autosomal dominant mitochondrial myopathy (Zeviani, M. et al., Nature 339, 309 (1989)). The non-D-loop regions with deletions were amplified by the polymerase chain reaction and the resulting fragments were subcloned and then sequenced. At least 12 deletions of different lengths in different sites were found. However, all the deletions were flanked by short direct repeats (4-12 base pairs).

Original languageEnglish
Pages (from-to)1352-1357
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume164
Issue number3
DOIs
Publication statusPublished - 1989 Nov 15
Externally publishedYes

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Mitochondrial Myopathies
Nucleic Acid Repetitive Sequences
Polymerase chain reaction
Mitochondrial DNA
Muscle
Peripheral Nervous System Diseases
Base Pairing
Muscles
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Multiple deletions in mitochondrial DNA at direct repeats of non-D-loop regions in cases of familial mitochondrial myopathy. / Yuzaki, Michisuke; Ohkoshi, Norio; Kanazawa, Ichiro; Kagawa, Yasuo; Ohta, Shigeo.

In: Biochemical and Biophysical Research Communications, Vol. 164, No. 3, 15.11.1989, p. 1352-1357.

Research output: Contribution to journalArticle

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