Multiple forms and a deficiency of uridine diphos-phate-glucuronosyltransferases in wistar rats

Michio Matsui, Fusako Nagai

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1 Citation (Scopus)

Abstract

Uridine diphosphate (UDP)-glucuronosyltransferase (GT) active on androsterone (AD) and 4-nitrophenol (NP) was solubilized from male rat liver microsomes of the Wistar strain. The precipitate obtained in the 60%-satd. ammonium sulfate was purified by diethyl-aminoethyl (DEAE)-cellulose chromatography and affinity chromatography on UDP-hexanolamine Sepharose 4B. DEAE-cellulose chromatography showed the existence of two peaks of GT active on AD and NP. Peak I was found in rats with the high-activity and low-activity phenotypes in terms of AD glucuronidation and had high NP-GT and low AD-GT activities. In contrast, peak II was found only in rats with the high-activity phenotype, corresponded to high AD-GT activity and had comparatively low NP-GT activities. The corresponding peak in rkts with the low-activity phenotype had only NP-GT activity. Comparison of Km values for AD obtained from microsomes and purified enzymes provides evidence that AD-GT isoenzyme should be deficient in Wistar rats with the low-activity phenotype and that AD glucuronidation should be catalyzed poorly by other GT isoenzyme in these rats.

Original languageEnglish
Pages (from-to)679-686
Number of pages8
JournalJournal of Pharmacobio-Dynamics
Volume8
Issue number8
DOIs
Publication statusPublished - 1985

Keywords

  • 4-nitrophenol
  • Wistar rat
  • androsterone
  • deficiency
  • isoenzyme
  • liver uridine diphosphate-glucuronosyltransferase
  • multiplicity

ASJC Scopus subject areas

  • Pharmacology

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