Multiple forms and a deficiency of uridine diphosphate- glucuronosyltransferases in Wistar rats

M. Matsui, F. Nagai

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Abstract

Uridine diphosphate (UDP)-glucuronosyltransferase (GT) active on androsterone (AD) and 4-nitrophenol (NP) was solubilized from male rat liver microsomes of the Wistar strain. The precipitate obtained in the 60%-statd. ammonium sulfate was purified by diethyl-aminoethyl (DEAE)-cellulose chromatography and affinity chromatography on UDP-hexanolamine Sepharose 4B. DEAE-cellulose chromatography showed the existence of two peaks of GT active on AD and NP. Peak I was found in rats with the high-activity and low-activity phenotypes in terms of AD glucuronidation and had high NP-GT and low AD-GT activities. In contrast, peak II was found only in rats with the high-activity phenotype, corresponded to high AD-GT activity and had comparatively low NP-GT activities. The corresponding peak in rats with the low-activity phenotype had only NP-GT activity. Comparison of K(m) values for AD obtained from microsomes and purified enzymes provides evidence that AD-GT isoenzyme should be deficient in Wistar rats with the low-activity phenotype and that AD glucuronidation should be catalyzed poorly by other GT isoenzyme in these rats.

Original languageEnglish
Pages (from-to)679-686
Number of pages8
JournalJournal of Pharmacobio-Dynamics
Volume8
Issue number8
Publication statusPublished - 1985

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Androsterone
Glucuronosyltransferase
Uridine Diphosphate
Wistar Rats
Phenotype
Isoenzymes
Chromatography
Ammonium Sulfate
Liver Microsomes
Microsomes
Affinity Chromatography
Sepharose
4-nitrophenol

ASJC Scopus subject areas

  • Pharmacology

Cite this

Multiple forms and a deficiency of uridine diphosphate- glucuronosyltransferases in Wistar rats. / Matsui, M.; Nagai, F.

In: Journal of Pharmacobio-Dynamics, Vol. 8, No. 8, 1985, p. 679-686.

Research output: Contribution to journalArticle

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