Multiple high-throughput analyses monitor the response of E. coli to perturbations

Nobuyoshi Ishii, Kenji Nakahigashi, Tomoya Baba, Martin Robert, Tomoyoshi Soga, Akio Kanai, Takashi Hirasawa, Miki Naba, Kenta Hirai, Aminul Hoque, Pei Yee Ho, Yuji Kakazu, Kaori Sugawara, Saori Igarashi, Satoshi Harada, Takeshi Masuda, Naoyuki Sugiyama, Takashi Togashi, Miki Hasegawa, Yuki TakaiKatsuyuki Yugi, Kazuharu Arakawa, Nayuta Iwata, Yoshihiro Toya, Yoichi Nakayama, Takaaki Nishioka, Kazuyuki Shimizu, Hirotada Mori, Masaru Tomita

Research output: Contribution to journalArticle

472 Citations (Scopus)

Abstract

Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.

Original languageEnglish
Pages (from-to)593-597
Number of pages5
JournalScience
Volume316
Issue number5824
DOIs
Publication statusPublished - 2007 Apr 27

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Escherichia coli
Metabolic Networks and Pathways
Enzymes
Messenger RNA
Growth
Genes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Multiple high-throughput analyses monitor the response of E. coli to perturbations. / Ishii, Nobuyoshi; Nakahigashi, Kenji; Baba, Tomoya; Robert, Martin; Soga, Tomoyoshi; Kanai, Akio; Hirasawa, Takashi; Naba, Miki; Hirai, Kenta; Hoque, Aminul; Ho, Pei Yee; Kakazu, Yuji; Sugawara, Kaori; Igarashi, Saori; Harada, Satoshi; Masuda, Takeshi; Sugiyama, Naoyuki; Togashi, Takashi; Hasegawa, Miki; Takai, Yuki; Yugi, Katsuyuki; Arakawa, Kazuharu; Iwata, Nayuta; Toya, Yoshihiro; Nakayama, Yoichi; Nishioka, Takaaki; Shimizu, Kazuyuki; Mori, Hirotada; Tomita, Masaru.

In: Science, Vol. 316, No. 5824, 27.04.2007, p. 593-597.

Research output: Contribution to journalArticle

Ishii, N, Nakahigashi, K, Baba, T, Robert, M, Soga, T, Kanai, A, Hirasawa, T, Naba, M, Hirai, K, Hoque, A, Ho, PY, Kakazu, Y, Sugawara, K, Igarashi, S, Harada, S, Masuda, T, Sugiyama, N, Togashi, T, Hasegawa, M, Takai, Y, Yugi, K, Arakawa, K, Iwata, N, Toya, Y, Nakayama, Y, Nishioka, T, Shimizu, K, Mori, H & Tomita, M 2007, 'Multiple high-throughput analyses monitor the response of E. coli to perturbations', Science, vol. 316, no. 5824, pp. 593-597. https://doi.org/10.1126/science.1132067
Ishii, Nobuyoshi ; Nakahigashi, Kenji ; Baba, Tomoya ; Robert, Martin ; Soga, Tomoyoshi ; Kanai, Akio ; Hirasawa, Takashi ; Naba, Miki ; Hirai, Kenta ; Hoque, Aminul ; Ho, Pei Yee ; Kakazu, Yuji ; Sugawara, Kaori ; Igarashi, Saori ; Harada, Satoshi ; Masuda, Takeshi ; Sugiyama, Naoyuki ; Togashi, Takashi ; Hasegawa, Miki ; Takai, Yuki ; Yugi, Katsuyuki ; Arakawa, Kazuharu ; Iwata, Nayuta ; Toya, Yoshihiro ; Nakayama, Yoichi ; Nishioka, Takaaki ; Shimizu, Kazuyuki ; Mori, Hirotada ; Tomita, Masaru. / Multiple high-throughput analyses monitor the response of E. coli to perturbations. In: Science. 2007 ; Vol. 316, No. 5824. pp. 593-597.
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