Multiple roles of vertebrate REV genes in DNA repair and recombination

Takashi Okada, Eiichiro Sonoda, Michio Yoshimura, Yoshiaki Kawano, Hideyuki Saya, Masaoki Kohzaki, Shunichi Takeda

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

In yeast, Rev1, Rev3, and Rev7 are involved in translesion synthesis over various kinds of DNA damage and spontaneous and UV-induced mutagenesis. Here, we disrupted Rev1, Rev3, and Rev7 in the chicken B-lymphocyte line DT40. REV1 -/- REV3-/- REV7-/- cells showed spontaneous cell death, chromosomal instability/fragility, and hypersensitivity to various genotoxic treatments as observed in each of the single mutants. Surprisingly, the triple-knockout cells showed a suppressed level of sister chromatid exchanges (SCEs), which may reflect postreplication repair events mediated by homologous recombination, while each single mutant showed an elevated SCE level. Furthermore, REV1-/- cells as well as triple mutants showed a decreased level of immunoglobulin gene conversion, suggesting participation of Rev1 in a recombination-based pathway. The present study gives us a new insight into cooperative function of three Rev molecules and the Polζ (Rev3-Rev7)-independent role of Rev1 in vertebrate cells.

Original languageEnglish
Pages (from-to)6103-6111
Number of pages9
JournalMolecular and Cellular Biology
Volume25
Issue number14
DOIs
Publication statusPublished - 2005 Jul
Externally publishedYes

Fingerprint

DNA Repair
Genetic Recombination
Vertebrates
Sister Chromatid Exchange
Genes
Chromosome Fragility
Gene Conversion
Chromosomal Instability
Immunoglobulin Genes
Homologous Recombination
Mutagenesis
DNA Damage
Chickens
Hypersensitivity
B-Lymphocytes
Cell Death
Yeasts

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Okada, T., Sonoda, E., Yoshimura, M., Kawano, Y., Saya, H., Kohzaki, M., & Takeda, S. (2005). Multiple roles of vertebrate REV genes in DNA repair and recombination. Molecular and Cellular Biology, 25(14), 6103-6111. https://doi.org/10.1128/MCB.25.14.6103-6111.2005

Multiple roles of vertebrate REV genes in DNA repair and recombination. / Okada, Takashi; Sonoda, Eiichiro; Yoshimura, Michio; Kawano, Yoshiaki; Saya, Hideyuki; Kohzaki, Masaoki; Takeda, Shunichi.

In: Molecular and Cellular Biology, Vol. 25, No. 14, 07.2005, p. 6103-6111.

Research output: Contribution to journalArticle

Okada, T, Sonoda, E, Yoshimura, M, Kawano, Y, Saya, H, Kohzaki, M & Takeda, S 2005, 'Multiple roles of vertebrate REV genes in DNA repair and recombination', Molecular and Cellular Biology, vol. 25, no. 14, pp. 6103-6111. https://doi.org/10.1128/MCB.25.14.6103-6111.2005
Okada, Takashi ; Sonoda, Eiichiro ; Yoshimura, Michio ; Kawano, Yoshiaki ; Saya, Hideyuki ; Kohzaki, Masaoki ; Takeda, Shunichi. / Multiple roles of vertebrate REV genes in DNA repair and recombination. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 14. pp. 6103-6111.
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