Multiple transcripts of human endothelin-A receptor gene detected by reverse transcription and the polymerase chain reaction

Yoshihiro Miyamoto, Takaaki Yoshimasa, Hiroshi Arai, Kazuhiko Takaya, Yoshihiro Ogawa, Hiroshi Itoh, Kazuwa Nakao

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To elucidate the regulatory mechanism of gene expression of the human endothelin-A receptor (hET-AR), we characterized the hET-AR transcripts using reverse transcription (RT) and polymerase chain reaction (PCR) analysis in a variety of human tissues. The RT-PCR using a set of primers in exons 2 and 5 showed two lower-molecular-weight transcripts in addition to the expected fragment. PCR cloning of these two novel transcripts revealed that these transcripts contain a 199-base pair (bp) and a 327-bp deletion compared with the previously described hET-AR cDNA, respectively. Comparison of their sequences with that of the hET-AR gene showed that the lacking sequences exactly correspond to exon 4 and exons 3 and 4, respectively, suggesting that these lower-molecular-weight ET-AR transcripts may result from alternative RNA splicing. Therefore, we isolated the cDNAs of novel transcripts of hET-AR that might be generated by alternative RNA splicing. These results suggest that the alternative RNA splicing might contribute to the regulation of ET-AR gene expression.

Original languageEnglish
Pages (from-to)S29-S31
JournalJournal of Cardiovascular Pharmacology
Volume26
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Keywords

  • Alternative splicing
  • Endothelin-A
  • Reverse transcription and polymerase chain reaction
  • receptor

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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