Multiple transcripts of human endothelin-A receptor gene detected by reverse transcription and the polymerase chain reaction

Y. Miyamoto, T. Yoshimasa, H. Arai, K. Takaya, Y. Ogawa, Hiroshi Itoh, K. Nakao

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To elucidate the regulatory mechanism of gene expression of the human endothelin-A receptor (hET-AR), we characterized the hET-AR transcripts using reverse transcription (RT) and polymerase chain reaction (PCR) analysis in a variety of human tissues. The RT-PCR using a set of primers in exons 2 and 5 showed two lower-molecular-weight transcripts in addition to the expected fragment. PCR cloning of these two novel transcripts revealed that these transcripts contain a 199-base pair (bp) and a 327-bp deletion compared with the previously described hET-AR cDNA, respectively. Comparison of their sequences with that of the hET-AR gene showed that the lacking sequences exactly correspond to exon 4 and exons 3 and 4, respectively, suggesting that these lower-molecular-weight ET-AR transcripts may result from alternative RNA splicing. Therefore, we isolated the cDNAs of novel transcripts of hET- AR that might be generated by alternative RNA splicing. These results suggest that the alternative RNA splicing might contribute to the regulation of ET- AR gene expression.

Original languageEnglish
JournalJournal of Cardiovascular Pharmacology
Volume26
Issue numberSUPPL. 3
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Endothelin A Receptors
Reverse Transcription
Alternative Splicing
Polymerase Chain Reaction
Exons
Genes
Base Pairing
Complementary DNA
Molecular Weight
Gene Expression
Regulator Genes
Organism Cloning

Keywords

  • Alternative splicing
  • Endothelin-A receptor
  • Reverse transcription and polymerase chain reaction

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

Multiple transcripts of human endothelin-A receptor gene detected by reverse transcription and the polymerase chain reaction. / Miyamoto, Y.; Yoshimasa, T.; Arai, H.; Takaya, K.; Ogawa, Y.; Itoh, Hiroshi; Nakao, K.

In: Journal of Cardiovascular Pharmacology, Vol. 26, No. SUPPL. 3, 1995.

Research output: Contribution to journalArticle

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AU - Miyamoto, Y.

AU - Yoshimasa, T.

AU - Arai, H.

AU - Takaya, K.

AU - Ogawa, Y.

AU - Itoh, Hiroshi

AU - Nakao, K.

PY - 1995

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N2 - To elucidate the regulatory mechanism of gene expression of the human endothelin-A receptor (hET-AR), we characterized the hET-AR transcripts using reverse transcription (RT) and polymerase chain reaction (PCR) analysis in a variety of human tissues. The RT-PCR using a set of primers in exons 2 and 5 showed two lower-molecular-weight transcripts in addition to the expected fragment. PCR cloning of these two novel transcripts revealed that these transcripts contain a 199-base pair (bp) and a 327-bp deletion compared with the previously described hET-AR cDNA, respectively. Comparison of their sequences with that of the hET-AR gene showed that the lacking sequences exactly correspond to exon 4 and exons 3 and 4, respectively, suggesting that these lower-molecular-weight ET-AR transcripts may result from alternative RNA splicing. Therefore, we isolated the cDNAs of novel transcripts of hET- AR that might be generated by alternative RNA splicing. These results suggest that the alternative RNA splicing might contribute to the regulation of ET- AR gene expression.

AB - To elucidate the regulatory mechanism of gene expression of the human endothelin-A receptor (hET-AR), we characterized the hET-AR transcripts using reverse transcription (RT) and polymerase chain reaction (PCR) analysis in a variety of human tissues. The RT-PCR using a set of primers in exons 2 and 5 showed two lower-molecular-weight transcripts in addition to the expected fragment. PCR cloning of these two novel transcripts revealed that these transcripts contain a 199-base pair (bp) and a 327-bp deletion compared with the previously described hET-AR cDNA, respectively. Comparison of their sequences with that of the hET-AR gene showed that the lacking sequences exactly correspond to exon 4 and exons 3 and 4, respectively, suggesting that these lower-molecular-weight ET-AR transcripts may result from alternative RNA splicing. Therefore, we isolated the cDNAs of novel transcripts of hET- AR that might be generated by alternative RNA splicing. These results suggest that the alternative RNA splicing might contribute to the regulation of ET- AR gene expression.

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