Multiplex PCR/liquid chromatography assay for screening of subtelomeric rearrangements

Toru Udaka, Issei Imoto, Yoshinori Aizu, Chiharu Torii, Kosuke Izumi, Rika Kosaki, Takao Takahashi, Shin Hayashi, Johji Inazawa, Kenjiro Kosaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In idiopathic or nonspecific mental retardation, the overall rate of cryptic subtelomeric rearrangements is estimated to be about 5%. Development of cost-effective screening for subtelomeric deletions would help clinical geneticists to make specific diagnoses in children with idiopathic mental retardation. Current screening modalities include fluorescence in situ hybridization (FISH) using subtelomeric probes and PCR-based quantitative analyses. Reductions in the cost and turnaround time will make the complete screening of subtelomeric rearrangements more widely used in clinical settings. Recently, a versatile method, called the multiplex PCR/liquid chromatography assay (MP/LC), was developed to assess copy numbers in this assay. Multiple genomic regions are amplified using unlabeled primers, then separated by ion-pair reversed-phase high-performance liquid chromatography. In the present study, we developed an MP/LC-based subtelomeric screening system that involves 21 multiple reactions and validated the protocol by analyzing 16 publicly available cell lines with known cytogenetic abnormalities involving at least one subtelomere per patient. To confirm the validity of the MP/LC method, we analyzed these cell lines concurrently with array-based comparative genomic hybridization (array-CGH), which gives higher resolution than the conventional G-banding technique. Among those 16 samples, the results from MP/LC and array-CGH agreed with each other perfectly. In 2 of the 16 samples, MP/LC correctly revealed subtelomeric duplications that were detected by array-CGH but were undetected by conventional cytogenetics, demonstrating the sensitivity of the MP/LC assay. This system is expected to be useful for making specific diagnoses and in genetic counseling for children with idiopathic mental retardation, a sizable fraction of whom have subtelomeric rearrangements.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalGenetic Testing
Volume11
Issue number3
DOIs
Publication statusPublished - 2007 Sep 1

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Multiplex Polymerase Chain Reaction
Liquid Chromatography
Comparative Genomic Hybridization
Intellectual Disability
Costs and Cost Analysis
Cell Line
Genetic Counseling
Reverse-Phase Chromatography
Fluorescence In Situ Hybridization
Cytogenetics
Chromosome Aberrations
High Pressure Liquid Chromatography
Ions
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

Multiplex PCR/liquid chromatography assay for screening of subtelomeric rearrangements. / Udaka, Toru; Imoto, Issei; Aizu, Yoshinori; Torii, Chiharu; Izumi, Kosuke; Kosaki, Rika; Takahashi, Takao; Hayashi, Shin; Inazawa, Johji; Kosaki, Kenjiro.

In: Genetic Testing, Vol. 11, No. 3, 01.09.2007, p. 241-248.

Research output: Contribution to journalArticle

Udaka, T, Imoto, I, Aizu, Y, Torii, C, Izumi, K, Kosaki, R, Takahashi, T, Hayashi, S, Inazawa, J & Kosaki, K 2007, 'Multiplex PCR/liquid chromatography assay for screening of subtelomeric rearrangements', Genetic Testing, vol. 11, no. 3, pp. 241-248. https://doi.org/10.1089/gte.2007.9993
Udaka, Toru ; Imoto, Issei ; Aizu, Yoshinori ; Torii, Chiharu ; Izumi, Kosuke ; Kosaki, Rika ; Takahashi, Takao ; Hayashi, Shin ; Inazawa, Johji ; Kosaki, Kenjiro. / Multiplex PCR/liquid chromatography assay for screening of subtelomeric rearrangements. In: Genetic Testing. 2007 ; Vol. 11, No. 3. pp. 241-248.
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