Multiscale tissue engineering for liver reconstruction

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The liver is a target of in vitro tissue engineering despite its capability to regenerate in vivo. The construction of liver tissues in vitro remains challenging. In this review, conventional 3D cultures of hepatocytes are first discussed. Recent advances in the 3D culturing of liver cells are then summarized in the context of in vitro liver tissue reconstruction at the micro-and macroscales. The application of microfluidics technology to liver tissue engineering has been introduced as a bottom-up approach performed at the microscale, whereas whole-organ bioengineering technology was introduced as a top-down approach performed at the macroscale. Mesoscale approaches are also discussed in considering the integration of micro-and macroscale approaches. Multiple parallel multiscale liver tissue engineering studies are ongoing; however, no tissue-engineered liver that is appropriate for clinical use has yet been realized. The integration of multiscale tissue engineering studies is essential for further understanding of liver reconstruction strategies.

Original languageEnglish
Pages (from-to)216-224
Number of pages9
JournalOrganogenesis
Volume10
Issue number2
DOIs
Publication statusPublished - 2014 Apr 1

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Tissue Engineering
Tissue engineering
Liver
Tissue
Technology
Bioengineering
Microfluidics
Hepatocytes
In Vitro Techniques

Keywords

  • 3D culture
  • Microfluidics
  • Tissue engineering
  • Vascularization

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Transplantation
  • Biomedical Engineering

Cite this

Multiscale tissue engineering for liver reconstruction. / Sudo, Ryo.

In: Organogenesis, Vol. 10, No. 2, 01.04.2014, p. 216-224.

Research output: Contribution to journalArticle

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