Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis

Noriyuki Kishi, Zhenyu Tang, Yusuke Maeda, Asami Hirai, Rong Mo, Mikiko Ito, Satoshi Suzuki, Keiko Nakao, Tsutomu Kinoshita, Tom Kadesch, Chi Chung Hui, Spyros Artavanis-Tsakonas, Hideyuki Okano, Kenji Matsuno

Research output: Contribution to journalArticle

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Abstract

Notch signaling plays an important role in cell-fate specification in multicellular organisms by regulating cell-cell communication. The Drosophila deltex gene encodes a modulator of the Notch pathway that has been shown to interact physically with the Ankyrin repeats of Notch. We isolated four distinct cDNAs corresponding to mouse homologs of deltex - mouse Deltex1 (MDTX1), mouse Deltex2 (MDTX2), mouse Deltex2ΔE (MDTX2ΔE), and mouse Deltex3 (MDTX3). Deduced amino acid sequences of these four cDNAs showed a high degree of similarity to Drosophila Deltex and its human homolog, DTX1 throughout their lengths, even though they possess distinct structural features. MDTX proteins formed homotypic and heterotypic multimers. We found that these genes were expressed in the central, peripheral nervous system and in the thymus, overlapping with those of mouse Notch1. In mammalian tissue culture cells, overexpression of any of the four mouse deltex homologs suppressed the transcriptional activity of E47, a basic helix-loop-helix (bHLH) protein, in a manner similar to suppression by an activated form of human Notch1 or human DTX1. In addition, overexpression of MDTX2 and MDTX2ΔE in C2C12 cells under differentiation-inducing conditions suppressed the expression of myogenin, one of the myogenic transcriptional factors; this was also similar to a previously reported activity of constitutively activated Notch. Furthermore, misexpression of any of the MDTX genes in Xenopus embryos resulted in an expansion of the region expressing the neural cell adhesion molecule (N-CAM) gene, a marker for the neuroepithelium. Collectively, our results suggest that these mouse deltex homologs are involved in vertebrate Notch signaling and regulation of neurogenesis.

Original languageEnglish
Pages (from-to)21-35
Number of pages15
JournalInternational Journal of Developmental Neuroscience
Volume19
Issue number1
DOIs
Publication statusPublished - 2001 Feb 1

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Neurogenesis
Vertebrates
Genes
Drosophila
Complementary DNA
Ankyrin Repeat
Myogenin
Neural Cell Adhesion Molecules
Peripheral Nervous System
Xenopus
Cell Communication
Thymus Gland
Cell Differentiation
Amino Acid Sequence
Proteins
Embryonic Structures
Central Nervous System
Cell Culture Techniques

Keywords

  • C2C12 cell
  • Cell signaling
  • Deltex
  • E47
  • Neurogenesis
  • Notch

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis. / Kishi, Noriyuki; Tang, Zhenyu; Maeda, Yusuke; Hirai, Asami; Mo, Rong; Ito, Mikiko; Suzuki, Satoshi; Nakao, Keiko; Kinoshita, Tsutomu; Kadesch, Tom; Hui, Chi Chung; Artavanis-Tsakonas, Spyros; Okano, Hideyuki; Matsuno, Kenji.

In: International Journal of Developmental Neuroscience, Vol. 19, No. 1, 01.02.2001, p. 21-35.

Research output: Contribution to journalArticle

Kishi, N, Tang, Z, Maeda, Y, Hirai, A, Mo, R, Ito, M, Suzuki, S, Nakao, K, Kinoshita, T, Kadesch, T, Hui, CC, Artavanis-Tsakonas, S, Okano, H & Matsuno, K 2001, 'Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis', International Journal of Developmental Neuroscience, vol. 19, no. 1, pp. 21-35. https://doi.org/10.1016/S0736-5748(00)00071-X
Kishi, Noriyuki ; Tang, Zhenyu ; Maeda, Yusuke ; Hirai, Asami ; Mo, Rong ; Ito, Mikiko ; Suzuki, Satoshi ; Nakao, Keiko ; Kinoshita, Tsutomu ; Kadesch, Tom ; Hui, Chi Chung ; Artavanis-Tsakonas, Spyros ; Okano, Hideyuki ; Matsuno, Kenji. / Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis. In: International Journal of Developmental Neuroscience. 2001 ; Vol. 19, No. 1. pp. 21-35.
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AU - Nakao, Keiko

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