Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization

Hiroyuki Yoshida, Aya Nagaoka, Sachiko Nakamura, Yoshinori Sugiyama, Yasunori Okada, Shintaro Inoue

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23 Citations (Scopus)

Abstract

Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.

Original languageEnglish
Pages (from-to)352-356
Number of pages5
JournalFEBS Open Bio
Volume3
DOIs
Publication statusPublished - 2013 Aug 1

Keywords

  • Catabolism
  • Extracellular matrix
  • Hyaluronan
  • KIAA1199

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Yoshida, H., Nagaoka, A., Nakamura, S., Sugiyama, Y., Okada, Y., & Inoue, S. (2013). Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization. FEBS Open Bio, 3, 352-356. https://doi.org/10.1016/j.fob.2013.08.003