Murine osteoblasts respond to LPS and IFN-γ similarly to macrophages

Kenta Maruyama, Gen Ichiro Sano, Koichi Matsuo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Osteoblasts are bone-forming mesenchymal cells, while macrophages are cells of hematopoietic origin responsible for innate immunity. Lipopolysaccharide (LPS) can induce tolerance in macrophages, whereas interferon (IFN)-γ can activate macrophages to produce cytokines, exert bactericidal effects, and present antigens. In this study, we examined such macrophagic phenotypes regulated by LPS and IFN-γ in murine osteoblasts. In both primary calvarial osteoblasts and osteoblastic MC3T3-E1 cells, LPS pretreatment resulted in reduced production of IL-6 in response to a subsequent LPS stimulation or to Salmonella infection, indicating the existence of LPS-induced tolerance in osteoblasts. Furthermore, IFN-γ treatment of MC3T3-E1 cells resulted in both enhanced IL-6 production in response to LPS and upregulation of major histocompatibility complex class II (MHC II). Following infection, Salmonella-containing vacuoles (SCVs) were formed in MC3T3-E1 cells, and IFN-γ pretreatment enhanced bactericidal effects on intracellular Salmonella. Taken together, these observations indicate that osteoblasts can exhibit a subset of phenotypes reminiscent of macrophages in the course of bacterial infection.

Original languageEnglish
Pages (from-to)454-460
Number of pages7
JournalJournal of Bone and Mineral Metabolism
Volume24
Issue number6
DOIs
Publication statusPublished - 2006 Nov 1

Keywords

  • IFN-γ
  • IL-6
  • LPS
  • MHC class II
  • Tolerance

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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