Musashi-1, an RNA-binding protein, is indispensable for survival of photoreceptors

Kanako Susaki, Jun Kaneko, Yuka Yamano, Kenta Nakamura, Wataru Inami, Taro Yoshikawa, Yoko Ozawa, Shinsuke Shibata, Osamu Matsuzaki, Hideyuki Okano, Chikafumi Chiba

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Musashi-1 (Msi1), an RNA-binding protein (RBP), has been postulated to play important roles in the maintenance of the stem-cell state, differentiation, and tumorigenesis. However, the expression and function of Msi1 in differentiated cells remain obscure. Here we show that Msi1 is expressed in mature photoreceptors and retinal pigment epithelium (RPE) cells, and is indispensable for the survival of photoreceptors. We found in the adult newt eye that Msi1 is expressed in all photoreceptors and RPE cells as well as in the retinal stem/progenitor cells in the ciliary marginal zone (CMZ). We found in the analyses of the newt normal and regenerating retinas that the expression profiles of the Msi1 transcripts and protein isoforms in the photoreceptors are different from those in the retinal stem/progenitor cells. Furthermore, we found that all photoreceptors and RPE cells of the adult mice also express Msi1, and that Msi1 knockout (Msi1-KO) results in degeneration of photoreceptors and a lack of a visual cycle protein RPE65 in the microvilli of RPE cells. Taken together, our current results demonstrate that the expression of Msi1 in mature photoreceptors and RPE cells is evolutionarily conserved, and that Msi1 bears essential functions for vision. Considering such an Msi1-KO phenotype in the retina, it is now reasonable to address whether defects of the Msi1 functions are responsible for inherited retinal diseases. Studying the regulation of Msi1 and the target RNAs of Msi1 in photoreceptors and RPE cells might contribute to fundamental and clinical studies of retinal degeneration.

Original languageEnglish
Pages (from-to)347-355
Number of pages9
JournalExperimental Eye Research
Volume88
Issue number3
DOIs
Publication statusPublished - 2009 Mar

Fingerprint

RNA-Binding Proteins
Retinal Pigment Epithelium
Stem Cells
Salamandridae
Retina
Retinal Diseases
Retinal Degeneration
Microvilli
Cell Differentiation
Protein Isoforms
Carcinogenesis
Maintenance
RNA
Phenotype

Keywords

  • Msi1-KO mouse
  • Musashi-1
  • newt
  • photoreceptor
  • regeneration
  • retinal pigment epithelium
  • retinitis pigmentosa
  • RNA-binding protein

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Susaki, K., Kaneko, J., Yamano, Y., Nakamura, K., Inami, W., Yoshikawa, T., ... Chiba, C. (2009). Musashi-1, an RNA-binding protein, is indispensable for survival of photoreceptors. Experimental Eye Research, 88(3), 347-355. https://doi.org/10.1016/j.exer.2008.06.019

Musashi-1, an RNA-binding protein, is indispensable for survival of photoreceptors. / Susaki, Kanako; Kaneko, Jun; Yamano, Yuka; Nakamura, Kenta; Inami, Wataru; Yoshikawa, Taro; Ozawa, Yoko; Shibata, Shinsuke; Matsuzaki, Osamu; Okano, Hideyuki; Chiba, Chikafumi.

In: Experimental Eye Research, Vol. 88, No. 3, 03.2009, p. 347-355.

Research output: Contribution to journalArticle

Susaki, K, Kaneko, J, Yamano, Y, Nakamura, K, Inami, W, Yoshikawa, T, Ozawa, Y, Shibata, S, Matsuzaki, O, Okano, H & Chiba, C 2009, 'Musashi-1, an RNA-binding protein, is indispensable for survival of photoreceptors', Experimental Eye Research, vol. 88, no. 3, pp. 347-355. https://doi.org/10.1016/j.exer.2008.06.019
Susaki, Kanako ; Kaneko, Jun ; Yamano, Yuka ; Nakamura, Kenta ; Inami, Wataru ; Yoshikawa, Taro ; Ozawa, Yoko ; Shibata, Shinsuke ; Matsuzaki, Osamu ; Okano, Hideyuki ; Chiba, Chikafumi. / Musashi-1, an RNA-binding protein, is indispensable for survival of photoreceptors. In: Experimental Eye Research. 2009 ; Vol. 88, No. 3. pp. 347-355.
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