Musashi and Seven in absentia downregulate Tramtrack through distinct mechanisms in Drosophila eye development

Yuki Hirota, Masataka Okabe, Takao Imai, Mitsuhiko Kurusu, Atsuyo Yamamoto, Sachiyo Miyao, Makoto Nakamura, Kazunobu Sawamoto, Hideyuki Okano

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have examined the roles played by the Drosophila neural RNA-binding protein Musashi (MSI) in eye development. MSI expression was observed in the nuclei of all photoreceptor cells (R1-R8). Although a msi loss-of-function mutation resulted in only weak abnormalities in photoreceptor differentiation, we found that the msi eye phenotype was significantly enhanced in a seven in absentia (sina) background. sina is known to be involved in the degradation of the Tramtrack (TTK) protein, leading to the specification of the R7 fate. We demonstrated that MSI also functions to regulate TTK expression. The sina msi mutants showed significantly high ectopic expression of TTK69 and failure in the determination of the R1, R6, and R7 fates. Other photoreceptor cells also failed to differentiate with abnormalities occurring late in the differentiation process. These results suggest that MSI and SINA function redundantly to downregulate TTK in developing photoreceptor cells. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)93-101
Number of pages9
JournalMechanisms of Development
Volume87
Issue number1-2
DOIs
Publication statusPublished - 1999 Sep 1
Externally publishedYes

Fingerprint

Photoreceptor Cells
Drosophila
Down-Regulation
RNA-Binding Proteins
Proteolysis
Phenotype
Mutation

Keywords

  • Drosophila
  • Eye development
  • Musashi
  • Photoreceptor cell
  • RNA-binding protein
  • Seven in absentia
  • Tramtrack

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Musashi and Seven in absentia downregulate Tramtrack through distinct mechanisms in Drosophila eye development. / Hirota, Yuki; Okabe, Masataka; Imai, Takao; Kurusu, Mitsuhiko; Yamamoto, Atsuyo; Miyao, Sachiyo; Nakamura, Makoto; Sawamoto, Kazunobu; Okano, Hideyuki.

In: Mechanisms of Development, Vol. 87, No. 1-2, 01.09.1999, p. 93-101.

Research output: Contribution to journalArticle

Hirota, Yuki ; Okabe, Masataka ; Imai, Takao ; Kurusu, Mitsuhiko ; Yamamoto, Atsuyo ; Miyao, Sachiyo ; Nakamura, Makoto ; Sawamoto, Kazunobu ; Okano, Hideyuki. / Musashi and Seven in absentia downregulate Tramtrack through distinct mechanisms in Drosophila eye development. In: Mechanisms of Development. 1999 ; Vol. 87, No. 1-2. pp. 93-101.
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