Mutation in hotfoot-4J mice results in retention of δ2 glutamate receptors in ER

Shinji Matsuda, Michisuke Yuzaki

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The orphan glutamate receptor δ2 is selectively expressed in Purkinje cells and plays a critical role in cerebellar function. Recently, the ataxia of hotfoot-4J (ho-4J) mice was shown to be caused by a 170-amino acid deletion in the N-terminal region of δ2 receptors. To understand δ2 receptor function, we characterized these mutant receptors (δ2ho) in Purkinje cells. Immunohistochemical staining showed that δ2ho receptors of the ho-4J homozygotes were abundantly expressed but localized to the Purkinje cell soma; in wild-type mice, δ2 receptors were predominantly present at distal dendrites of Purkinje cells. In addition, δ2ho receptors of the ho-4J mice were sensitive to endoglycosidase H, a finding suggesting that δ2ho receptors were not transported beyond the endoplasmic reticulum (ER) or cis-Golgi apparatus. To gain further insights into the mechanisms of this phenomenon, we characterized δ2ho receptors in transfected HEK293 cells. δ2ho receptors expressed in HEK293 cells were also sensitive to endoglycosidase H. Immunohistochemical staining showed that δ2ho receptors colocalized with proteins retained in the ER. Furthermore, δ2ho receptors were not labelled by membrane-impermeable biotinylation reagents. Coimmunoprecipitation assays showed that the intermolecular interaction of δ2ho receptors was significantly weaker than those of wild-type δ2 receptors, a finding suggesting that the ho-4J region is involved in oligomerization of δ2 receptors. Thus, δ2ho receptors were retained in the ER, probably by the quality control mechanism that detects unstable oligomers. We conclude that the absence of δ2 receptors on the cell surface by failed transport from the ER of Purkinje cells causes ataxia.

Original languageEnglish
Pages (from-to)1507-1516
Number of pages10
JournalEuropean Journal of Neuroscience
Volume16
Issue number8
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Purkinje Cells
Glutamate Receptors
Endoplasmic Reticulum
Mutation
HEK293 Cells
Glycoside Hydrolases
Ataxia
Staining and Labeling
Biotinylation
Homozygote
Cell Surface Receptors
Golgi Apparatus
Carisoprodol
Dendrites
Quality Control
Amino Acids
Membranes
Proteins

Keywords

  • Assembly
  • Ataxia
  • Cerebellum
  • Mutant
  • Purkinje cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mutation in hotfoot-4J mice results in retention of δ2 glutamate receptors in ER. / Matsuda, Shinji; Yuzaki, Michisuke.

In: European Journal of Neuroscience, Vol. 16, No. 8, 2002, p. 1507-1516.

Research output: Contribution to journalArticle

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