Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model

Takako Osaki, Tomoko Hanawa, Taki Manzoku, Minoru Fukuda, Hayato Kawakami, Hidekazu Suzuki, Hiroyuki Yamaguchi, Xu Yan, Haruhiko Taguchi, Satoshi Kurata, Shigeru Kamiya

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42 Citations (Scopus)

Abstract

Helicobacter pylori is associated with gastric disorders in humans and some experimental animals, and possesses the luxS/type 2 autoinducer (Al-2) system. The effects of a specific luxS mutation on the characteristics of H. pylori were examined. On 0·3% agar medium, motility of H. pylori HPKY08 (luxS::cat) was significantly lower than that of wild-type H. pylori TK1402, The luxS-complemented strain HPKY21 exhibited motility comparable to that of H. pylori TK1402. It was shown that the luxS/Al-2 system plays an important role in H. pylori motility. The luxS mutant exhibited a reduced infection rate relative to the wild-type parent strain TK1402 in a Mongolian gerbil model. At 3 months after oral inoculation, lower numbers of H. pylori were detected by semi-quantitative real-time reverse transcription PCR (qRT-PCR) in luxS - mutant-infected gerbils than in TK1402-infected gerbils. Gastric inflammation and increased antibody titre for H. pylori were observed in TK1402-infected gerbils only.

Original languageEnglish
Pages (from-to)1477-1485
Number of pages9
JournalJournal of Medical Microbiology
Volume55
Issue number11
DOIs
Publication statusPublished - 2006 Nov 1

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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    Osaki, T., Hanawa, T., Manzoku, T., Fukuda, M., Kawakami, H., Suzuki, H., Yamaguchi, H., Yan, X., Taguchi, H., Kurata, S., & Kamiya, S. (2006). Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model. Journal of Medical Microbiology, 55(11), 1477-1485. https://doi.org/10.1099/jmm.0.46660-0