Mutation of Y925F in focal adhesion kinase (FAK) suppresses melanoma cell proliferation and metastasis

Tomonori Kaneda, Yoshiko Sonoda, Kumi Ando, Takaharu Suzuki, Yasuhiro Sasaki, Tomoyuki Oshio, Megumi Tago, Tadashi Kasahara

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42 Citations (Scopus)

Abstract

This study focused on the role of focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase important for many cellular processes, in the proliferation, adhesion, and invasion of melanoma cells in vitro and in vivo. We found that the Y925F-mutation of FAK in B16F10 melanoma cells suppressed metastasis in an experimental model, which correlated well with decreased extracellular matrix dependent proliferative capability, adhesive, migrational, and invasive capabilities. Transduction of the mutation Y925F resulted in a down-regulation of the phosphorylation of Erk, the expression of VEGF, and the association of FAK with paxillin. The results provide clear evidence that 925Y of FAK is critical for melanoma metastasis and this phosphorylation site will be an anti-metastatic target.

Original languageEnglish
Pages (from-to)354-361
Number of pages8
JournalCancer Letters
Volume270
Issue number2
DOIs
Publication statusPublished - 2008 Nov 8

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Keywords

  • B16F10 cells
  • Erk
  • FAK
  • Metastasis
  • Paxillin
  • Y925F-mutation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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