Mutation pattern of the p53 gene as a diagnostic marker for multiple hepatocellular carcinoma

Tatsuya Oda, Hitoshi Tsuda, Aldo Scarpa, Michiie Sakamoto, Setsuo Hirohashi

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Hepatocellular carcinoma, sometimes shows multiple tumor nodules, therefore poses a problem of differential diagnosis between cancers of multifocal and those of metastatic origin. Conventionally, pathological criteria have been used for this purpose, but these are largely subjective. In order to facilitate more objective differential diagnosis of multiple hepatocellular carcinoma, we used the pattern of mutation of the p53 gene as a marker for each tumor nodule. We studied 58 nodules from 26 cases of multiple hepatocellular carcinoma using polymerase chain reaction-single strand conformation polymorphism analysis, a simple method for detecting mutations. p53 gene mutations were detected in 65% (17 of 26) of cases. The internodule mutation patterns were heterogeneous in 11 cases and homogeneous in 6, enabling a multifocal origin to be diagnosed in the former and a metastatic origin in the latter at the genetic level. Moreover, the origin of recurrent tumors was determined from the mutation pattern. It is concluded that analysis of p53 mutations seems to be useful for differentiating the origin of multiple cancers, since the information it yields is essentially objective.

Original languageEnglish
Pages (from-to)3674-3678
Number of pages5
JournalCancer Research
Volume52
Issue number13
Publication statusPublished - 1992 Jul 1
Externally publishedYes

Fingerprint

p53 Genes
Hepatocellular Carcinoma
Mutation
Neoplasms
Differential Diagnosis
Tumor Biomarkers
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Mutation pattern of the p53 gene as a diagnostic marker for multiple hepatocellular carcinoma. / Oda, Tatsuya; Tsuda, Hitoshi; Scarpa, Aldo; Sakamoto, Michiie; Hirohashi, Setsuo.

In: Cancer Research, Vol. 52, No. 13, 01.07.1992, p. 3674-3678.

Research output: Contribution to journalArticle

Oda, T, Tsuda, H, Scarpa, A, Sakamoto, M & Hirohashi, S 1992, 'Mutation pattern of the p53 gene as a diagnostic marker for multiple hepatocellular carcinoma', Cancer Research, vol. 52, no. 13, pp. 3674-3678.
Oda, Tatsuya ; Tsuda, Hitoshi ; Scarpa, Aldo ; Sakamoto, Michiie ; Hirohashi, Setsuo. / Mutation pattern of the p53 gene as a diagnostic marker for multiple hepatocellular carcinoma. In: Cancer Research. 1992 ; Vol. 52, No. 13. pp. 3674-3678.
@article{15bf3345ca29474986a851f693bc0448,
title = "Mutation pattern of the p53 gene as a diagnostic marker for multiple hepatocellular carcinoma",
abstract = "Hepatocellular carcinoma, sometimes shows multiple tumor nodules, therefore poses a problem of differential diagnosis between cancers of multifocal and those of metastatic origin. Conventionally, pathological criteria have been used for this purpose, but these are largely subjective. In order to facilitate more objective differential diagnosis of multiple hepatocellular carcinoma, we used the pattern of mutation of the p53 gene as a marker for each tumor nodule. We studied 58 nodules from 26 cases of multiple hepatocellular carcinoma using polymerase chain reaction-single strand conformation polymorphism analysis, a simple method for detecting mutations. p53 gene mutations were detected in 65{\%} (17 of 26) of cases. The internodule mutation patterns were heterogeneous in 11 cases and homogeneous in 6, enabling a multifocal origin to be diagnosed in the former and a metastatic origin in the latter at the genetic level. Moreover, the origin of recurrent tumors was determined from the mutation pattern. It is concluded that analysis of p53 mutations seems to be useful for differentiating the origin of multiple cancers, since the information it yields is essentially objective.",
author = "Tatsuya Oda and Hitoshi Tsuda and Aldo Scarpa and Michiie Sakamoto and Setsuo Hirohashi",
year = "1992",
month = "7",
day = "1",
language = "English",
volume = "52",
pages = "3674--3678",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "13",

}

TY - JOUR

T1 - Mutation pattern of the p53 gene as a diagnostic marker for multiple hepatocellular carcinoma

AU - Oda, Tatsuya

AU - Tsuda, Hitoshi

AU - Scarpa, Aldo

AU - Sakamoto, Michiie

AU - Hirohashi, Setsuo

PY - 1992/7/1

Y1 - 1992/7/1

N2 - Hepatocellular carcinoma, sometimes shows multiple tumor nodules, therefore poses a problem of differential diagnosis between cancers of multifocal and those of metastatic origin. Conventionally, pathological criteria have been used for this purpose, but these are largely subjective. In order to facilitate more objective differential diagnosis of multiple hepatocellular carcinoma, we used the pattern of mutation of the p53 gene as a marker for each tumor nodule. We studied 58 nodules from 26 cases of multiple hepatocellular carcinoma using polymerase chain reaction-single strand conformation polymorphism analysis, a simple method for detecting mutations. p53 gene mutations were detected in 65% (17 of 26) of cases. The internodule mutation patterns were heterogeneous in 11 cases and homogeneous in 6, enabling a multifocal origin to be diagnosed in the former and a metastatic origin in the latter at the genetic level. Moreover, the origin of recurrent tumors was determined from the mutation pattern. It is concluded that analysis of p53 mutations seems to be useful for differentiating the origin of multiple cancers, since the information it yields is essentially objective.

AB - Hepatocellular carcinoma, sometimes shows multiple tumor nodules, therefore poses a problem of differential diagnosis between cancers of multifocal and those of metastatic origin. Conventionally, pathological criteria have been used for this purpose, but these are largely subjective. In order to facilitate more objective differential diagnosis of multiple hepatocellular carcinoma, we used the pattern of mutation of the p53 gene as a marker for each tumor nodule. We studied 58 nodules from 26 cases of multiple hepatocellular carcinoma using polymerase chain reaction-single strand conformation polymorphism analysis, a simple method for detecting mutations. p53 gene mutations were detected in 65% (17 of 26) of cases. The internodule mutation patterns were heterogeneous in 11 cases and homogeneous in 6, enabling a multifocal origin to be diagnosed in the former and a metastatic origin in the latter at the genetic level. Moreover, the origin of recurrent tumors was determined from the mutation pattern. It is concluded that analysis of p53 mutations seems to be useful for differentiating the origin of multiple cancers, since the information it yields is essentially objective.

UR - http://www.scopus.com/inward/record.url?scp=0026632451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026632451&partnerID=8YFLogxK

M3 - Article

C2 - 1319827

AN - SCOPUS:0026632451

VL - 52

SP - 3674

EP - 3678

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 13

ER -