Mutation Pattern of the p53 Gene as a Diagnostic Marker for Multiple Hepatocellular Carcinoma

Tatsuya Oda, Hitoshi Tsuda, Aldo Scarpa, Michiie Sakamoto, Setsuo Hirohashi

Research output: Contribution to journalArticlepeer-review

166 Citations (Scopus)

Abstract

Hepatocellular carcinoma, sometimes shows multiple tumor nodules, therefore poses a problem of differential diagnosis between cancers of multifocal and those of metastatic origin. Conventionally, pathological criteria have been used for this purpose, but these are largely subjective. In order to facilitate more objective differential diagnosis of multiple hepatocellular carcinoma, we used the pattern of mutation of the p53 gene as a marker for each tumor nodule. We studied 58 nodules from 26 cases of multiple hepatocellular carcinoma using polymerase chain reaction-single strand conformation polymorphism analysis, a simple method for detecting mutations. p53 gene mutations were detected in 65% (17 of 26) of cases. The internodule mutation patterns were heterogeneous in 11 cases and homogeneous in 6, enabling a multifocal origin to be diagnosed in the former and a metastatic origin in the latter at the genetic level. Moreover, the origin of recurrent tumors was determined from the mutation pattern. It is concluded that analysis of p53 mutations seems to be useful for differentiating the origin of multiple cancers, since the information it yields is essentially objective.

Original languageEnglish
Pages (from-to)3674-3678
Number of pages5
JournalCancer Research
Volume52
Issue number13
Publication statusPublished - 1992 Jul
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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