Mutational analysis of β-catenin and the RAS-RAF signalling pathway in early flat-type colorectal tumours

Masashi Mikami, Katsuhiko Nosho, Hiroyuki Yamamoto, Taiga Takahashi, Tadateru Maehata, Hiroaki Taniguchi, Yasushi Adachi, Akimichi Imamura, Masahiro Fujita, Masao Hosokawa, Fumio Itoh, Kohzoh Imai, Yasuhisa Shinomura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Morphologically, early colorectal tumours can be divided into two groups, protruded-type and flat-type. However, little is known about genetic mechanisms of the latter. We investigated mutations of β-catenin, KRAS, BRAF, and PIK3CA in 310 early colorectal tumours. β-catenin mutation was detected in 7.1% of 310 tumours. β-catenin mutation was detected in a significantly higher percentage of flat-type tumours with depressed areas (4/17, 23.5%) than in other tumours (18/293, 6.1%; p = 0.0246). KRAS, BRAF, and PIK3CA mutations were detected in 21.6%, 5.4%, and 1.0% of 310 tumours, respectively. Concomitant mutations of β-catenin and KRAS or BRAF were detected in seven tumours. Mutation of at least one gene was detected in a significantly higher percentage of flat-type tumour tissues (75/193, 38.9%) than in protruded-type tumour tissues (25/117, 21.4%; p = 0.0014), and it was correlated significantly with size (p = 0.0001). In conclusion, β-catenin mutation seemed to play an important role in flat-type tumours, especially in those with depressed areas. The genetic abnormalities could arise and accumulate in the early stage of colorectal tumourigenesis, and seem to contribute to the development of flat-type tumour.

Original languageEnglish
Pages (from-to)3065-3072
Number of pages8
JournalEuropean Journal of Cancer
Volume42
Issue number17
DOIs
Publication statusPublished - 2006 Nov
Externally publishedYes

Fingerprint

Catenins
Colorectal Neoplasms
Mutation
Neoplasms

Keywords

  • β-catenin
  • BRAF
  • Flat-type colorectal tumours
  • KRAS
  • PIK3CA
  • RAS-RAF signalling pathway

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Mutational analysis of β-catenin and the RAS-RAF signalling pathway in early flat-type colorectal tumours. / Mikami, Masashi; Nosho, Katsuhiko; Yamamoto, Hiroyuki; Takahashi, Taiga; Maehata, Tadateru; Taniguchi, Hiroaki; Adachi, Yasushi; Imamura, Akimichi; Fujita, Masahiro; Hosokawa, Masao; Itoh, Fumio; Imai, Kohzoh; Shinomura, Yasuhisa.

In: European Journal of Cancer, Vol. 42, No. 17, 11.2006, p. 3065-3072.

Research output: Contribution to journalArticle

Mikami, M, Nosho, K, Yamamoto, H, Takahashi, T, Maehata, T, Taniguchi, H, Adachi, Y, Imamura, A, Fujita, M, Hosokawa, M, Itoh, F, Imai, K & Shinomura, Y 2006, 'Mutational analysis of β-catenin and the RAS-RAF signalling pathway in early flat-type colorectal tumours', European Journal of Cancer, vol. 42, no. 17, pp. 3065-3072. https://doi.org/10.1016/j.ejca.2006.06.029
Mikami, Masashi ; Nosho, Katsuhiko ; Yamamoto, Hiroyuki ; Takahashi, Taiga ; Maehata, Tadateru ; Taniguchi, Hiroaki ; Adachi, Yasushi ; Imamura, Akimichi ; Fujita, Masahiro ; Hosokawa, Masao ; Itoh, Fumio ; Imai, Kohzoh ; Shinomura, Yasuhisa. / Mutational analysis of β-catenin and the RAS-RAF signalling pathway in early flat-type colorectal tumours. In: European Journal of Cancer. 2006 ; Vol. 42, No. 17. pp. 3065-3072.
@article{6753d0cc951c4c5d8d5eba94a3454a34,
title = "Mutational analysis of β-catenin and the RAS-RAF signalling pathway in early flat-type colorectal tumours",
abstract = "Morphologically, early colorectal tumours can be divided into two groups, protruded-type and flat-type. However, little is known about genetic mechanisms of the latter. We investigated mutations of β-catenin, KRAS, BRAF, and PIK3CA in 310 early colorectal tumours. β-catenin mutation was detected in 7.1{\%} of 310 tumours. β-catenin mutation was detected in a significantly higher percentage of flat-type tumours with depressed areas (4/17, 23.5{\%}) than in other tumours (18/293, 6.1{\%}; p = 0.0246). KRAS, BRAF, and PIK3CA mutations were detected in 21.6{\%}, 5.4{\%}, and 1.0{\%} of 310 tumours, respectively. Concomitant mutations of β-catenin and KRAS or BRAF were detected in seven tumours. Mutation of at least one gene was detected in a significantly higher percentage of flat-type tumour tissues (75/193, 38.9{\%}) than in protruded-type tumour tissues (25/117, 21.4{\%}; p = 0.0014), and it was correlated significantly with size (p = 0.0001). In conclusion, β-catenin mutation seemed to play an important role in flat-type tumours, especially in those with depressed areas. The genetic abnormalities could arise and accumulate in the early stage of colorectal tumourigenesis, and seem to contribute to the development of flat-type tumour.",
keywords = "β-catenin, BRAF, Flat-type colorectal tumours, KRAS, PIK3CA, RAS-RAF signalling pathway",
author = "Masashi Mikami and Katsuhiko Nosho and Hiroyuki Yamamoto and Taiga Takahashi and Tadateru Maehata and Hiroaki Taniguchi and Yasushi Adachi and Akimichi Imamura and Masahiro Fujita and Masao Hosokawa and Fumio Itoh and Kohzoh Imai and Yasuhisa Shinomura",
year = "2006",
month = "11",
doi = "10.1016/j.ejca.2006.06.029",
language = "English",
volume = "42",
pages = "3065--3072",
journal = "European Journal of Cancer",
issn = "0959-8049",
publisher = "Elsevier Limited",
number = "17",

}

TY - JOUR

T1 - Mutational analysis of β-catenin and the RAS-RAF signalling pathway in early flat-type colorectal tumours

AU - Mikami, Masashi

AU - Nosho, Katsuhiko

AU - Yamamoto, Hiroyuki

AU - Takahashi, Taiga

AU - Maehata, Tadateru

AU - Taniguchi, Hiroaki

AU - Adachi, Yasushi

AU - Imamura, Akimichi

AU - Fujita, Masahiro

AU - Hosokawa, Masao

AU - Itoh, Fumio

AU - Imai, Kohzoh

AU - Shinomura, Yasuhisa

PY - 2006/11

Y1 - 2006/11

N2 - Morphologically, early colorectal tumours can be divided into two groups, protruded-type and flat-type. However, little is known about genetic mechanisms of the latter. We investigated mutations of β-catenin, KRAS, BRAF, and PIK3CA in 310 early colorectal tumours. β-catenin mutation was detected in 7.1% of 310 tumours. β-catenin mutation was detected in a significantly higher percentage of flat-type tumours with depressed areas (4/17, 23.5%) than in other tumours (18/293, 6.1%; p = 0.0246). KRAS, BRAF, and PIK3CA mutations were detected in 21.6%, 5.4%, and 1.0% of 310 tumours, respectively. Concomitant mutations of β-catenin and KRAS or BRAF were detected in seven tumours. Mutation of at least one gene was detected in a significantly higher percentage of flat-type tumour tissues (75/193, 38.9%) than in protruded-type tumour tissues (25/117, 21.4%; p = 0.0014), and it was correlated significantly with size (p = 0.0001). In conclusion, β-catenin mutation seemed to play an important role in flat-type tumours, especially in those with depressed areas. The genetic abnormalities could arise and accumulate in the early stage of colorectal tumourigenesis, and seem to contribute to the development of flat-type tumour.

AB - Morphologically, early colorectal tumours can be divided into two groups, protruded-type and flat-type. However, little is known about genetic mechanisms of the latter. We investigated mutations of β-catenin, KRAS, BRAF, and PIK3CA in 310 early colorectal tumours. β-catenin mutation was detected in 7.1% of 310 tumours. β-catenin mutation was detected in a significantly higher percentage of flat-type tumours with depressed areas (4/17, 23.5%) than in other tumours (18/293, 6.1%; p = 0.0246). KRAS, BRAF, and PIK3CA mutations were detected in 21.6%, 5.4%, and 1.0% of 310 tumours, respectively. Concomitant mutations of β-catenin and KRAS or BRAF were detected in seven tumours. Mutation of at least one gene was detected in a significantly higher percentage of flat-type tumour tissues (75/193, 38.9%) than in protruded-type tumour tissues (25/117, 21.4%; p = 0.0014), and it was correlated significantly with size (p = 0.0001). In conclusion, β-catenin mutation seemed to play an important role in flat-type tumours, especially in those with depressed areas. The genetic abnormalities could arise and accumulate in the early stage of colorectal tumourigenesis, and seem to contribute to the development of flat-type tumour.

KW - β-catenin

KW - BRAF

KW - Flat-type colorectal tumours

KW - KRAS

KW - PIK3CA

KW - RAS-RAF signalling pathway

UR - http://www.scopus.com/inward/record.url?scp=33750514803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750514803&partnerID=8YFLogxK

U2 - 10.1016/j.ejca.2006.06.029

DO - 10.1016/j.ejca.2006.06.029

M3 - Article

C2 - 17011185

AN - SCOPUS:33750514803

VL - 42

SP - 3065

EP - 3072

JO - European Journal of Cancer

JF - European Journal of Cancer

SN - 0959-8049

IS - 17

ER -