Mutational analysis of growth arrest and cellular localization of human immunodeficiency virus type 1 Vpr in the budding yeast, Saccharomyces cerevisiae

Junko Nakazawa, Nobumoto Watanabe, Masaya Imoto, Hiroyuki Osada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Viral protein R (Vpr), one of the accessory gene products of human immunodeficiency virus type 1 (HIV-1), is responsible for the incorporation of a viral genome into the nucleus upon infection. Vpr also arrests the cell cycle and induces apoptosis in infected cells. Similarly, in yeast, Vpr localizes in the nucleus and shows growth inhibitory activity; however, the molecular mechanism of growth inhibition remains unknown. To elucidate this mechanism, several point mutations of Vpr, which are known to perturb several phenotypes of Vpr in mammalian cells, were introduced in the budding yeast, Saccharomyces cerevisiae. For the first time, we found that growth inhibition by Vpr occurred independently of intracellular localization in yeast, as has previously been reported in mammals. We also indentified several amino acid residues, the mutation of which cancels growth inhibitory activity, and/or alters localization, both in yeast and mammalian cells, suggesting the importance of these residues for the phenotypes.

Original languageEnglish
Pages (from-to)245-256
Number of pages12
JournalJournal of General and Applied Microbiology
Volume51
Issue number4
DOIs
Publication statusPublished - 2005 Oct 3

Keywords

  • Growth inhibition
  • Saccharomyces cerevisiae
  • Vpr

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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