Mutations in the Plk gene lead to instability of Plk protein in human tumour cell lines

Siro Simizu, H. Osada

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

It has been established that mutations in Drosophila Polo cause abnormalities in mitosis. In human cells, maximal Plk activity is reached in the M phase of the cell cycle, and the function of Plk is therefore considered to be required for mitotic cellular events such as spindle formation, chromosome segregation and cytokinesis. Microinjection of anti-Plk antibody into living cells has been found to induce a mitotic abnormality that contributes to the generation of aneuploidy, and this is an important finding in relation to tumour development. Indeed, previous studies have shown that the level of expression of a mitotic checkpoint gene, hsMAD2, is reduced and that another checkpoint gene, BUB1, is mutated in certain human cancer cells.

Original languageEnglish
Pages (from-to)852-854
Number of pages3
JournalNature Cell Biology
Volume2
Issue number11
DOIs
Publication statusPublished - 2000
Externally publishedYes

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Tumor Cell Line
Mutation
M Phase Cell Cycle Checkpoints
Genes
Chromosome Segregation
Proteins
Cytokinesis
Microinjections
Aneuploidy
Mitosis
Cell Division
Drosophila
Anti-Idiotypic Antibodies
Neoplasms
Cell Cycle

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mutations in the Plk gene lead to instability of Plk protein in human tumour cell lines. / Simizu, Siro; Osada, H.

In: Nature Cell Biology, Vol. 2, No. 11, 2000, p. 852-854.

Research output: Contribution to journalArticle

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