Mycotrienin II, a translation inhibitor that prevents ICAM-1 expression induced by pro-inflammatory cytokines

Yuriko Yamada, Etsu Tashiro, Shigeru Taketani, Masaya Imoto, Takao Kataoka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin-1α (IL-1α), trigger the activation of the transcription factor nuclear factor-B, a molecule that induces the expression of a variety of genes, including intercellular adhesion molecule-1 (ICAM-1). Here, we report that mycotrienin II, a member of the triene-ansamycin group, inhibited the cell-surface ICAM-1 expression induced by TNF-α more strongly than that induced by IL-1α in human lung carcinoma A549 cells. Mycotrienin II was found to inhibit protein synthesis in intact living cells, as well as in cell-free translation systems. Among translation inhibitors tested, acetoxycycloheximide and anisomycin, but neither puromycin nor emetine, inhibited the TNF-α-induced ICAM-1 expression at lower concentrations than the IL-1α-induced ICAM-1 expression. Several compounds of the triene-ansamycin group (that is, mycotrienin I, trienomycin A, trierixin, quinotrierixin and quinotrierixin HQ) also inhibited ICAM-1 expression, as well as cell-free translation in a manner similar to mycotrienin II. These results indicate that mycotrienin II is a direct inhibitor of translation, thereby inhibiting ICAM-1 expression induced by pro-inflammatory cytokines.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalJournal of Antibiotics
Volume64
Issue number5
DOIs
Publication statusPublished - 2011 May

Fingerprint

Intercellular Adhesion Molecule-1
Cytokines
Interleukin-1
Rifabutin
Tumor Necrosis Factor-alpha
Emetine
Anisomycin
Puromycin
Cell-Free System
mycotrienin II
Transcription Factors
Carcinoma
Lung
Genes
Proteins

Keywords

  • ICAM-1
  • IL-1α
  • mycotrienin II
  • NF-κB
  • pro-inflammatory cytokine
  • TNF-α
  • triene-ansamycin

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Mycotrienin II, a translation inhibitor that prevents ICAM-1 expression induced by pro-inflammatory cytokines. / Yamada, Yuriko; Tashiro, Etsu; Taketani, Shigeru; Imoto, Masaya; Kataoka, Takao.

In: Journal of Antibiotics, Vol. 64, No. 5, 05.2011, p. 361-366.

Research output: Contribution to journalArticle

@article{926bb19b60df490188c821aa8c32e976,
title = "Mycotrienin II, a translation inhibitor that prevents ICAM-1 expression induced by pro-inflammatory cytokines",
abstract = "Pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin-1α (IL-1α), trigger the activation of the transcription factor nuclear factor-B, a molecule that induces the expression of a variety of genes, including intercellular adhesion molecule-1 (ICAM-1). Here, we report that mycotrienin II, a member of the triene-ansamycin group, inhibited the cell-surface ICAM-1 expression induced by TNF-α more strongly than that induced by IL-1α in human lung carcinoma A549 cells. Mycotrienin II was found to inhibit protein synthesis in intact living cells, as well as in cell-free translation systems. Among translation inhibitors tested, acetoxycycloheximide and anisomycin, but neither puromycin nor emetine, inhibited the TNF-α-induced ICAM-1 expression at lower concentrations than the IL-1α-induced ICAM-1 expression. Several compounds of the triene-ansamycin group (that is, mycotrienin I, trienomycin A, trierixin, quinotrierixin and quinotrierixin HQ) also inhibited ICAM-1 expression, as well as cell-free translation in a manner similar to mycotrienin II. These results indicate that mycotrienin II is a direct inhibitor of translation, thereby inhibiting ICAM-1 expression induced by pro-inflammatory cytokines.",
keywords = "ICAM-1, IL-1α, mycotrienin II, NF-κB, pro-inflammatory cytokine, TNF-α, triene-ansamycin",
author = "Yuriko Yamada and Etsu Tashiro and Shigeru Taketani and Masaya Imoto and Takao Kataoka",
year = "2011",
month = "5",
doi = "10.1038/ja.2011.23",
language = "English",
volume = "64",
pages = "361--366",
journal = "Journal of Antibiotics",
issn = "0021-8820",
publisher = "Japan Antibiotics Research Association",
number = "5",

}

TY - JOUR

T1 - Mycotrienin II, a translation inhibitor that prevents ICAM-1 expression induced by pro-inflammatory cytokines

AU - Yamada, Yuriko

AU - Tashiro, Etsu

AU - Taketani, Shigeru

AU - Imoto, Masaya

AU - Kataoka, Takao

PY - 2011/5

Y1 - 2011/5

N2 - Pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin-1α (IL-1α), trigger the activation of the transcription factor nuclear factor-B, a molecule that induces the expression of a variety of genes, including intercellular adhesion molecule-1 (ICAM-1). Here, we report that mycotrienin II, a member of the triene-ansamycin group, inhibited the cell-surface ICAM-1 expression induced by TNF-α more strongly than that induced by IL-1α in human lung carcinoma A549 cells. Mycotrienin II was found to inhibit protein synthesis in intact living cells, as well as in cell-free translation systems. Among translation inhibitors tested, acetoxycycloheximide and anisomycin, but neither puromycin nor emetine, inhibited the TNF-α-induced ICAM-1 expression at lower concentrations than the IL-1α-induced ICAM-1 expression. Several compounds of the triene-ansamycin group (that is, mycotrienin I, trienomycin A, trierixin, quinotrierixin and quinotrierixin HQ) also inhibited ICAM-1 expression, as well as cell-free translation in a manner similar to mycotrienin II. These results indicate that mycotrienin II is a direct inhibitor of translation, thereby inhibiting ICAM-1 expression induced by pro-inflammatory cytokines.

AB - Pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin-1α (IL-1α), trigger the activation of the transcription factor nuclear factor-B, a molecule that induces the expression of a variety of genes, including intercellular adhesion molecule-1 (ICAM-1). Here, we report that mycotrienin II, a member of the triene-ansamycin group, inhibited the cell-surface ICAM-1 expression induced by TNF-α more strongly than that induced by IL-1α in human lung carcinoma A549 cells. Mycotrienin II was found to inhibit protein synthesis in intact living cells, as well as in cell-free translation systems. Among translation inhibitors tested, acetoxycycloheximide and anisomycin, but neither puromycin nor emetine, inhibited the TNF-α-induced ICAM-1 expression at lower concentrations than the IL-1α-induced ICAM-1 expression. Several compounds of the triene-ansamycin group (that is, mycotrienin I, trienomycin A, trierixin, quinotrierixin and quinotrierixin HQ) also inhibited ICAM-1 expression, as well as cell-free translation in a manner similar to mycotrienin II. These results indicate that mycotrienin II is a direct inhibitor of translation, thereby inhibiting ICAM-1 expression induced by pro-inflammatory cytokines.

KW - ICAM-1

KW - IL-1α

KW - mycotrienin II

KW - NF-κB

KW - pro-inflammatory cytokine

KW - TNF-α

KW - triene-ansamycin

UR - http://www.scopus.com/inward/record.url?scp=79957592423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79957592423&partnerID=8YFLogxK

U2 - 10.1038/ja.2011.23

DO - 10.1038/ja.2011.23

M3 - Article

VL - 64

SP - 361

EP - 366

JO - Journal of Antibiotics

JF - Journal of Antibiotics

SN - 0021-8820

IS - 5

ER -