Myelin basic protein gene and the function of antisense RNA in its repression in myelin-deficient mutant mouse

Hideyuki Okano, Jun Aruga, Toshiyuki Nakagawa, Chiyo Shiota, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The myelin-deficient (mld) mouse is an autosomal recessive mutant characterized by hypomyelination of the CNS due to reduced expression of the myelin basic protein (MBP) gene. In the mld mutant, the MBP gene is duplicated in tandem. One gene is intact, but a large portion is inverted upstream of the other copy, and its transcription yields the antisense RNA. This antisense RNA was shown to be localized in the nucleus and to form an RNA:RNA duplex with sense RNA. These findings suggested that inhibition of transport from the nucleus or selective degradation of the duplex is responsible for the reduced expression of the MBP gene in the mld mutant. The mechanism of gene rearrangement at the MBP locus was also characterized. Cosmid clones encompassing whole MBP gene loci from control and mld genomic DNA libraries were isolated. The recombination points indicated that the duplication and inversion observed in mld occurred due to nonhomologous recombination.

Original languageEnglish
Pages (from-to)560-567
Number of pages8
JournalJournal of Neurochemistry
Volume56
Issue number2
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Antisense RNA
Myelin Basic Protein
Myelin Sheath
Genes
RNA
Genetic Recombination
Cosmids
Internal-External Control
Genomic Library
Gene Rearrangement
Gene Library
Transcription
Clone Cells
Degradation

Keywords

  • Antisense RNA
  • Gene rearrangement
  • Myelin basic protein
  • Myelin-deficient (mld) mutant
  • RNA:RNA duplex

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Myelin basic protein gene and the function of antisense RNA in its repression in myelin-deficient mutant mouse. / Okano, Hideyuki; Aruga, Jun; Nakagawa, Toshiyuki; Shiota, Chiyo; Mikoshiba, Katsuhiko.

In: Journal of Neurochemistry, Vol. 56, No. 2, 1991, p. 560-567.

Research output: Contribution to journalArticle

Okano, Hideyuki ; Aruga, Jun ; Nakagawa, Toshiyuki ; Shiota, Chiyo ; Mikoshiba, Katsuhiko. / Myelin basic protein gene and the function of antisense RNA in its repression in myelin-deficient mutant mouse. In: Journal of Neurochemistry. 1991 ; Vol. 56, No. 2. pp. 560-567.
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