Myeloid and erythroid lineage expression of haemopoietic progenitors derived from an abnormal clone in erythroleukaemia

T. Suda, Y. Sato, Y. Furukawa, M. Eguchi, M. Saito, Y. Miura

Research output: Contribution to journalArticle

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Abstract

To clarify the lineage involvement of haemopoietic progenitor cells in erythroleukaemia, the morphology and chromosomes of single colonies from a patient with erythroleukaemia were analysed simultaneously. The cytogenetic analysis of bone marrow cells revealed two clones; 44,XY-7,-12,-17,del(5)(q31),+Mar and 43,XY,-7,-12,-17,-19,del(5)(q31),+Mar. Of 40 metaphases examined, there were 34 and six of these clones, respectively. Bone marrow mononuclear cells were plated at 5 x 104/ml in methylcellulose medium containing phytohaemagglutinin-stimulated leucocyte conditioned medium and erythropoietin. Seventeen colonies, i.e. nine blast cell colonies, four myeloid (Sudan black B-positive) colonies, and four erythroid (benzidine-positive) colonies contained analysable metaphases, yielding 102 metaphases in total. Except for chromosome random loss, the karyotype within a colony remained constant. All three types of colonies showed an abnormal clone; 44,XY,-7,-12,-17,del(5)(q31),+Mar. From these findings, it is concluded that myeloid and erythroid lineages in erythroleukaemia were derived from the same abnormal clone.

Original languageEnglish
Pages (from-to)647-656
Number of pages10
JournalBritish Journal of Haematology
Volume64
Issue number4
Publication statusPublished - 1986

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Leukemia, Erythroblastic, Acute
Metaphase
Clone Cells
Bone Marrow Cells
Chromosomes
Mars
Methylcellulose
Cytogenetic Analysis
Phytohemagglutinins
Conditioned Culture Medium
Erythropoietin
Karyotype
Leukocytes
Stem Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Myeloid and erythroid lineage expression of haemopoietic progenitors derived from an abnormal clone in erythroleukaemia. / Suda, T.; Sato, Y.; Furukawa, Y.; Eguchi, M.; Saito, M.; Miura, Y.

In: British Journal of Haematology, Vol. 64, No. 4, 1986, p. 647-656.

Research output: Contribution to journalArticle

Suda, T, Sato, Y, Furukawa, Y, Eguchi, M, Saito, M & Miura, Y 1986, 'Myeloid and erythroid lineage expression of haemopoietic progenitors derived from an abnormal clone in erythroleukaemia', British Journal of Haematology, vol. 64, no. 4, pp. 647-656.
Suda, T. ; Sato, Y. ; Furukawa, Y. ; Eguchi, M. ; Saito, M. ; Miura, Y. / Myeloid and erythroid lineage expression of haemopoietic progenitors derived from an abnormal clone in erythroleukaemia. In: British Journal of Haematology. 1986 ; Vol. 64, No. 4. pp. 647-656.
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AU - Saito, M.

AU - Miura, Y.

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AB - To clarify the lineage involvement of haemopoietic progenitor cells in erythroleukaemia, the morphology and chromosomes of single colonies from a patient with erythroleukaemia were analysed simultaneously. The cytogenetic analysis of bone marrow cells revealed two clones; 44,XY-7,-12,-17,del(5)(q31),+Mar and 43,XY,-7,-12,-17,-19,del(5)(q31),+Mar. Of 40 metaphases examined, there were 34 and six of these clones, respectively. Bone marrow mononuclear cells were plated at 5 x 104/ml in methylcellulose medium containing phytohaemagglutinin-stimulated leucocyte conditioned medium and erythropoietin. Seventeen colonies, i.e. nine blast cell colonies, four myeloid (Sudan black B-positive) colonies, and four erythroid (benzidine-positive) colonies contained analysable metaphases, yielding 102 metaphases in total. Except for chromosome random loss, the karyotype within a colony remained constant. All three types of colonies showed an abnormal clone; 44,XY,-7,-12,-17,del(5)(q31),+Mar. From these findings, it is concluded that myeloid and erythroid lineages in erythroleukaemia were derived from the same abnormal clone.

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