Myoblast adhesion and proliferation on biodegradable polymer films with femtosecond laser-fabricated micro through-holes

Izumi Takayama, Naonari Kondo, Stefan Kalies, Alexander Heisterkamp, Mitsuhiro Terakawa

Research output: Contribution to journalArticle

Abstract

Controlling cell adhesion and cell differentiation is necessary to fabricate a tissue with arbitrary properties for tissue engineering applications. A substrate with a porous structure as a cell scaffold allows the diffusion of the cell culture medium through the scaffold. In this work, we show that the femtosecond laser fabricated micro through-holes in biodegradable polymer films, enhance myoblast adhesion, and accelerates proliferation and differentiation. ChR2-C2C12 and UT-C2C12 cells were seeded on the films with micro through-holes each fabricated by a single femtosecond laser pulse. Cell adhesion was enhanced on films with holes fabricated by laser irradiation. In addition, cell proliferation was accelerated on films with micro through-holes that penetrate the film, compared to on films with micro craters that do not penetrate the film. On films with arrays consisting of micro through-holes, cells aligned along the arrays and cell fusion was enhanced, indicating the acceleration of cell differentiation.

Original languageEnglish
Article numbere202000037
JournalJournal of Biophotonics
Volume13
Issue number7
DOIs
Publication statusPublished - 2020 Jul 1

Keywords

  • C2C12 cells
  • cell adhesion
  • cell differentiation
  • cell proliferation
  • femtosecond laser ablation
  • micro through-holes

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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