Myocardial electrical conduction blockade time dominated by irradiance on photodynamic reaction: In vitro and in silico study

Emiyu Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The time for electrical conduction blockade induced by a photodynamic reaction was studied on a myocardial cell wire in vitro and an in silico simulation model was constructed to understand the necessary time for electrical conduction blockade for the wire. Vulnerable state of the cells on a laser interaction would be an unstable and undesirable state since the cells might progress to completely damaged or repaired to change significantly therapeutic effect. So that in silico model, which can calculate the vulnerable cell state, is needed. Understanding an immediate electrical conduction blockade is needed for our proposed new methodology for tachyarrhythmia catheter ablation applying a photodynamic reaction. We studied the electrical conduction blockade occurrence on the electrical conduction wire made of cultured myocardial cells in a line shape and constructed in silico model based on this experimental data. The intracellular Ca2+ ion concentrations were obtained using Fluo-4 AM dye under a confocal laser microscope. A cross-correlation function was used for the electrical conduction blockade judgment. The photodynamic reaction was performed under the confocal microscopy with 3-120 mW/cm2 in irradiance by the diode laser with 663 nm in wavelength. We obtained that the time for the electrical conduction blockade decreased with the irradiance increasing. We constructed a simulation model composed of three states; living cells, vulnerable cells, and blocked cells, using the obtained experimental data and we found the rate constant by an optimization using a conjugate gradient method.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXIX
PublisherSPIE
Volume10492
ISBN (Electronic)9781510614697
DOIs
Publication statusPublished - 2018 Jan 1
EventOptical Interactions with Tissue and Cells XXIX 2018 - San Francisco, United States
Duration: 2018 Jan 292018 Jan 30

Other

OtherOptical Interactions with Tissue and Cells XXIX 2018
CountryUnited States
CitySan Francisco
Period18/1/2918/1/30

Keywords

  • electrical conduction
  • In silico model
  • myocardial cell
  • photosensitization reaction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Ogawa, E., & Arai, T. (2018). Myocardial electrical conduction blockade time dominated by irradiance on photodynamic reaction: In vitro and in silico study. In Optical Interactions with Tissue and Cells XXIX (Vol. 10492). [104920E] SPIE. https://doi.org/10.1117/12.2289319