Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart

Yoh Arita, Yoshikazu Nakaoka, Taichi Matsunaga, Hiroyasu Kidoya, Kohei Yamamizu, Yuichiro Arima, Takahiro Kataoka-Hashimoto, Kuniyasu Ikeoka, Taku Yasui, Takeshi Masaki, Kaori Yamamoto, Kaori Higuchi, Jin Sung Park, Manabu Shirai, Koichi Nishiyama, Hiroyuki Yamagishi, Kinya Otsu, Hiroki Kurihara, Takashi Minami, Keiko Yamauchi-Takihara & 6 others Gou Y. Koh, Naoki Mochizuki, Nobuyuki Takakura, Yasushi Sakata, Jun K. Yamashita, Issei Komuro

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The origin and developmental mechanisms underlying coronary vessels are not fully elucidated. Here we show that myocardium-derived angiopoietin-1 (Ang1) is essential for coronary vein formation in the developing heart. Cardiomyocyte-specific Ang1 deletion results in defective formation of the subepicardial coronary veins, but had no significant effect on the formation of intramyocardial coronary arteries. The endothelial cells (ECs) of the sinus venosus (SV) are heterogeneous population, composed of APJ-positive and APJ-negative ECs. Among these, the APJ-negative ECs migrate from the SV into the atrial and ventricular myocardium in Ang1-dependent manner. In addition, Ang1 may positively regulate venous differentiation of the subepicardial APJ-negative ECs in the heart. Consistently, in vitro experiments show that Ang1 indeed promotes venous differentiation of the immature ECs. Collectively, our results indicate that myocardial Ang1 positively regulates coronary vein formation presumably by promoting the proliferation, migration and differentiation of immature ECs derived from the SV.

Original languageEnglish
Article number4552
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 2014 Jul 29

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Angiopoietin-1
myocardium
Endothelial cells
veins
sinuses
Coronary Vessels
Myocardium
Endothelial Cells
deletion
arteries
vessels
Cardiac Myocytes
Population

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Arita, Y., Nakaoka, Y., Matsunaga, T., Kidoya, H., Yamamizu, K., Arima, Y., ... Komuro, I. (2014). Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart. Nature Communications, 5, [4552]. https://doi.org/10.1038/ncomms5552

Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart. / Arita, Yoh; Nakaoka, Yoshikazu; Matsunaga, Taichi; Kidoya, Hiroyasu; Yamamizu, Kohei; Arima, Yuichiro; Kataoka-Hashimoto, Takahiro; Ikeoka, Kuniyasu; Yasui, Taku; Masaki, Takeshi; Yamamoto, Kaori; Higuchi, Kaori; Park, Jin Sung; Shirai, Manabu; Nishiyama, Koichi; Yamagishi, Hiroyuki; Otsu, Kinya; Kurihara, Hiroki; Minami, Takashi; Yamauchi-Takihara, Keiko; Koh, Gou Y.; Mochizuki, Naoki; Takakura, Nobuyuki; Sakata, Yasushi; Yamashita, Jun K.; Komuro, Issei.

In: Nature Communications, Vol. 5, 4552, 29.07.2014.

Research output: Contribution to journalArticle

Arita, Y, Nakaoka, Y, Matsunaga, T, Kidoya, H, Yamamizu, K, Arima, Y, Kataoka-Hashimoto, T, Ikeoka, K, Yasui, T, Masaki, T, Yamamoto, K, Higuchi, K, Park, JS, Shirai, M, Nishiyama, K, Yamagishi, H, Otsu, K, Kurihara, H, Minami, T, Yamauchi-Takihara, K, Koh, GY, Mochizuki, N, Takakura, N, Sakata, Y, Yamashita, JK & Komuro, I 2014, 'Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart', Nature Communications, vol. 5, 4552. https://doi.org/10.1038/ncomms5552
Arita, Yoh ; Nakaoka, Yoshikazu ; Matsunaga, Taichi ; Kidoya, Hiroyasu ; Yamamizu, Kohei ; Arima, Yuichiro ; Kataoka-Hashimoto, Takahiro ; Ikeoka, Kuniyasu ; Yasui, Taku ; Masaki, Takeshi ; Yamamoto, Kaori ; Higuchi, Kaori ; Park, Jin Sung ; Shirai, Manabu ; Nishiyama, Koichi ; Yamagishi, Hiroyuki ; Otsu, Kinya ; Kurihara, Hiroki ; Minami, Takashi ; Yamauchi-Takihara, Keiko ; Koh, Gou Y. ; Mochizuki, Naoki ; Takakura, Nobuyuki ; Sakata, Yasushi ; Yamashita, Jun K. ; Komuro, Issei. / Myocardium-derived angiopoietin-1 is essential for coronary vein formation in the developing heart. In: Nature Communications. 2014 ; Vol. 5.
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