Myocilin expression in the astrocytes of the optic nerve head

Setsuko Noda, Yukihiko Mashima, Minoru Obazawa, Ryo Kubota, Yoshihisa Oguchi, Jun Kudoh, Shinsei Minoshima, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We investigated the expression of myocilin in the optic nerve head of porcine eyes by Western blotting and immunohistochemical staining. Myocilin was localized in the nucleus, centrosome, glial filament, mitochondria, and some parts of the cell membranes of the astrocytes. Myocilin was also detected at the edge-feet portion of the processes of astrocytes adjacent to the inner limiting membrane and blood vessel wall. The astrocytes are the major cell population in the optic nerve head, contributing to the architecture of the nerve axon and blood vessels. Therefore, myocilin gene mutation and change of myocilin protein are likely to affect the architecture of the optic nerve head and induce various forms of glaucomatous optic nerve damage. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)1129-1135
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume276
Issue number3
DOIs
Publication statusPublished - 2000 Oct 5

Keywords

  • Astrocyte
  • Centrosome
  • Glaucoma
  • Immunoelectromicroscopic analysis
  • Myocilin
  • Optic nerve head
  • Porcine eye

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Noda, S., Mashima, Y., Obazawa, M., Kubota, R., Oguchi, Y., Kudoh, J., Minoshima, S., & Shimizu, N. (2000). Myocilin expression in the astrocytes of the optic nerve head. Biochemical and Biophysical Research Communications, 276(3), 1129-1135. https://doi.org/10.1006/bbrc.2000.3591