N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer

Akira Seko, Fumio Kataoka, Daisuke Aoki, Masaru Sakamoto, Toshiaki Nakamura, Masayuki Hatae, Suguru Yonezawa, Katsuko Yamashita

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

N-Acetylglucosamine 6-O-sulfotransferase-2 (GlcNAc6ST2) is ectopically expressed in ovarian mucinous and clear cell adenocarcinoma [Kanoh et al., Glycoconj J 23:453-460, 2006]. Here we studied whether GlcNAc6ST2 protein can be detected in sera from patients with gynecological cancers and could serve as a tumor marker. First, we created a monoclonal antibody and polyclonal antiserum against GlcNAc6ST2. These antibodies were specific for GlcNAc6ST2, as shown by Western blot analysis and immunoprecipitation. Using these antibodies, we constructed a sandwich ELISA method for detecting GlcNAc6ST2 in the serum. GlcNAc6ST2 provided lower positive rates for ovarian cancer than CA125, but higher positive rates for uterine cervical and corpus cancer than SCC antigens and CA125, respectively. A significantly higher percentage of stage I uterine cervical and corpus cancers were positive for GlcNAc6ST2 than for SCC antigens and CA125, respectively. GlcNAc6ST2 could therefore be a good serological marker for detecting early-stage uterine cervical and corpus cancers.

Original languageEnglish
Pages (from-to)1065-1073
Number of pages9
JournalGlycoconjugate Journal
Volume26
Issue number8
DOIs
Publication statusPublished - 2009 Nov

Fingerprint

CA-125 Antigen
Sulfotransferases
Acetylglucosamine
Tumor Biomarkers
Uterine Cervical Neoplasms
Antibodies
Clear Cell Adenocarcinoma
Immune Sera
Monoclonal Antibodies
Serum
Immunoprecipitation
Ovarian Neoplasms
Western Blotting
Enzyme-Linked Immunosorbent Assay
Proteins
squamous cell carcinoma-related antigen
Neoplasms

Keywords

  • Ovarian cancer
  • Sandwich ELISA
  • Sulfotransferase
  • Tumor marker
  • Uterine cervical cancer
  • Uterine corpus cancer

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer. / Seko, Akira; Kataoka, Fumio; Aoki, Daisuke; Sakamoto, Masaru; Nakamura, Toshiaki; Hatae, Masayuki; Yonezawa, Suguru; Yamashita, Katsuko.

In: Glycoconjugate Journal, Vol. 26, No. 8, 11.2009, p. 1065-1073.

Research output: Contribution to journalArticle

Seko, Akira ; Kataoka, Fumio ; Aoki, Daisuke ; Sakamoto, Masaru ; Nakamura, Toshiaki ; Hatae, Masayuki ; Yonezawa, Suguru ; Yamashita, Katsuko. / N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer. In: Glycoconjugate Journal. 2009 ; Vol. 26, No. 8. pp. 1065-1073.
@article{ab50f58dcd184498b34e8254a1f7b852,
title = "N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer",
abstract = "N-Acetylglucosamine 6-O-sulfotransferase-2 (GlcNAc6ST2) is ectopically expressed in ovarian mucinous and clear cell adenocarcinoma [Kanoh et al., Glycoconj J 23:453-460, 2006]. Here we studied whether GlcNAc6ST2 protein can be detected in sera from patients with gynecological cancers and could serve as a tumor marker. First, we created a monoclonal antibody and polyclonal antiserum against GlcNAc6ST2. These antibodies were specific for GlcNAc6ST2, as shown by Western blot analysis and immunoprecipitation. Using these antibodies, we constructed a sandwich ELISA method for detecting GlcNAc6ST2 in the serum. GlcNAc6ST2 provided lower positive rates for ovarian cancer than CA125, but higher positive rates for uterine cervical and corpus cancer than SCC antigens and CA125, respectively. A significantly higher percentage of stage I uterine cervical and corpus cancers were positive for GlcNAc6ST2 than for SCC antigens and CA125, respectively. GlcNAc6ST2 could therefore be a good serological marker for detecting early-stage uterine cervical and corpus cancers.",
keywords = "Ovarian cancer, Sandwich ELISA, Sulfotransferase, Tumor marker, Uterine cervical cancer, Uterine corpus cancer",
author = "Akira Seko and Fumio Kataoka and Daisuke Aoki and Masaru Sakamoto and Toshiaki Nakamura and Masayuki Hatae and Suguru Yonezawa and Katsuko Yamashita",
year = "2009",
month = "11",
doi = "10.1007/s10719-008-9227-4",
language = "English",
volume = "26",
pages = "1065--1073",
journal = "Glycoconjugate Journal",
issn = "0282-0080",
publisher = "Springer Netherlands",
number = "8",

}

TY - JOUR

T1 - N-Acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer

AU - Seko, Akira

AU - Kataoka, Fumio

AU - Aoki, Daisuke

AU - Sakamoto, Masaru

AU - Nakamura, Toshiaki

AU - Hatae, Masayuki

AU - Yonezawa, Suguru

AU - Yamashita, Katsuko

PY - 2009/11

Y1 - 2009/11

N2 - N-Acetylglucosamine 6-O-sulfotransferase-2 (GlcNAc6ST2) is ectopically expressed in ovarian mucinous and clear cell adenocarcinoma [Kanoh et al., Glycoconj J 23:453-460, 2006]. Here we studied whether GlcNAc6ST2 protein can be detected in sera from patients with gynecological cancers and could serve as a tumor marker. First, we created a monoclonal antibody and polyclonal antiserum against GlcNAc6ST2. These antibodies were specific for GlcNAc6ST2, as shown by Western blot analysis and immunoprecipitation. Using these antibodies, we constructed a sandwich ELISA method for detecting GlcNAc6ST2 in the serum. GlcNAc6ST2 provided lower positive rates for ovarian cancer than CA125, but higher positive rates for uterine cervical and corpus cancer than SCC antigens and CA125, respectively. A significantly higher percentage of stage I uterine cervical and corpus cancers were positive for GlcNAc6ST2 than for SCC antigens and CA125, respectively. GlcNAc6ST2 could therefore be a good serological marker for detecting early-stage uterine cervical and corpus cancers.

AB - N-Acetylglucosamine 6-O-sulfotransferase-2 (GlcNAc6ST2) is ectopically expressed in ovarian mucinous and clear cell adenocarcinoma [Kanoh et al., Glycoconj J 23:453-460, 2006]. Here we studied whether GlcNAc6ST2 protein can be detected in sera from patients with gynecological cancers and could serve as a tumor marker. First, we created a monoclonal antibody and polyclonal antiserum against GlcNAc6ST2. These antibodies were specific for GlcNAc6ST2, as shown by Western blot analysis and immunoprecipitation. Using these antibodies, we constructed a sandwich ELISA method for detecting GlcNAc6ST2 in the serum. GlcNAc6ST2 provided lower positive rates for ovarian cancer than CA125, but higher positive rates for uterine cervical and corpus cancer than SCC antigens and CA125, respectively. A significantly higher percentage of stage I uterine cervical and corpus cancers were positive for GlcNAc6ST2 than for SCC antigens and CA125, respectively. GlcNAc6ST2 could therefore be a good serological marker for detecting early-stage uterine cervical and corpus cancers.

KW - Ovarian cancer

KW - Sandwich ELISA

KW - Sulfotransferase

KW - Tumor marker

KW - Uterine cervical cancer

KW - Uterine corpus cancer

UR - http://www.scopus.com/inward/record.url?scp=72649087617&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72649087617&partnerID=8YFLogxK

U2 - 10.1007/s10719-008-9227-4

DO - 10.1007/s10719-008-9227-4

M3 - Article

C2 - 19156517

AN - SCOPUS:72649087617

VL - 26

SP - 1065

EP - 1073

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

SN - 0282-0080

IS - 8

ER -