NADH-dependent dehydroascorbate reductase in the rabbit lens

Ichiko Akatsuka, Masayasu Bando, Hajime Obazawa, Mikako Oka, Makoto Takehana, Shizuko Kobayashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The present investigation demonstrates the existence of NADH-dependent dehydroascorbate (DHA) reductase activity in the soluble fraction of the rabbit lens. This DHA reductase was specific for NADH, and its apparent Km values for DHA and NADH were 5.7 mM and 4.0 μM, respectively. In a gel filtration of the lens soluble fraction on a Sephadex G-75 superfine column, the NADH-dependent DHA reductase activity was eluted at the oligomeric βL1-crystallin fraction, which may also contain λ-crystallin (a rabbit-specific crystallin). Furthermore, about 80% of protein fractions separated from the βL1-crystallin fraction by DEAE-cellulose ion-exchange column chromatography exhibited DHA reductase activity. In the SDS-PAGE analysis of the protein fractions with DHA reductase activity, 32-33, 27 and 25 kDa protein subunit s were commonly identified. These results suggest that oligomers of β-crystallin and/or λ-crystallin subunits may be associated with the DHA reductase activity. The present paper also discusses that the function of the reductase may be to enhance the antiphotoxidation capacity of the lens.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalTokai Journal of Experimental and Clinical Medicine
Volume26
Issue number1
Publication statusPublished - 2001 Apr

Fingerprint

glutathione dehydrogenase (ascorbate)
Crystallins
NAD
Lenses
Rabbits
DEAE-Cellulose Chromatography
Ion Exchange Chromatography
Protein Subunits
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Oxidoreductases
Proteins

Keywords

  • Dehydroascorbate reductase
  • Enzyme/Crystallin, antiphotoxidation
  • NADH-specific
  • Rabbit lens

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Akatsuka, I., Bando, M., Obazawa, H., Oka, M., Takehana, M., & Kobayashi, S. (2001). NADH-dependent dehydroascorbate reductase in the rabbit lens. Tokai Journal of Experimental and Clinical Medicine, 26(1), 25-32.

NADH-dependent dehydroascorbate reductase in the rabbit lens. / Akatsuka, Ichiko; Bando, Masayasu; Obazawa, Hajime; Oka, Mikako; Takehana, Makoto; Kobayashi, Shizuko.

In: Tokai Journal of Experimental and Clinical Medicine, Vol. 26, No. 1, 04.2001, p. 25-32.

Research output: Contribution to journalArticle

Akatsuka, I, Bando, M, Obazawa, H, Oka, M, Takehana, M & Kobayashi, S 2001, 'NADH-dependent dehydroascorbate reductase in the rabbit lens', Tokai Journal of Experimental and Clinical Medicine, vol. 26, no. 1, pp. 25-32.
Akatsuka I, Bando M, Obazawa H, Oka M, Takehana M, Kobayashi S. NADH-dependent dehydroascorbate reductase in the rabbit lens. Tokai Journal of Experimental and Clinical Medicine. 2001 Apr;26(1):25-32.
Akatsuka, Ichiko ; Bando, Masayasu ; Obazawa, Hajime ; Oka, Mikako ; Takehana, Makoto ; Kobayashi, Shizuko. / NADH-dependent dehydroascorbate reductase in the rabbit lens. In: Tokai Journal of Experimental and Clinical Medicine. 2001 ; Vol. 26, No. 1. pp. 25-32.
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