Naive-like ESRRB+ iPSCs with the Capacity for Rapid Neural Differentiation

Fumihiko Kisa, Seiji Shiozawa, Keisuke Oda, Sho Yoshimatsu, Mari Nakamura, Ikuko Koya, Kenji Kawai, Sadafumi Suzuki, Hideyuki Okano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Several groups have reported the existence of a form of pluripotency that resembles that of mouse embryonic stem cells (mESCs), i.e., a naive state, in human pluripotent stem cells; however, the characteristics vary between reports. The nuclear receptor ESRRB is expressed in mESCs and plays a significant role in their self-renewal, but its expression has not been observed in most naive-like human induced pluripotent stem cells (hiPSCs). In this study, we modified several methods for converting hiPSCs into a naive state through the transgenic expression of several reprogramming factors. The resulting cells express the components of the core transcriptional network of mESCs, including ESRRB, at high levels, which suggests the existence of naive-state hiPSCs that are similar to mESCs. We also demonstrate that these cells differentiate more readily into neural cells than do conventional hiPSCs. These features may be beneficial for their use in disease modeling and regenerative medicine. Kisa et al. modified several methods for converting human induced pluripotent stem cells (hiPSCs) into a naive state, a form of pluripotency that exists in mouse embryonic stem cells (ESCs). Converted cells express components of the core transcriptional network upregulated in mouse ESCs, including ESRRB. They also show that these cells differentiate more readily into neural cells than do conventional hiPSCs.

Original languageEnglish
JournalStem Cell Reports
DOIs
Publication statusAccepted/In press - 2017 Jan 18

Keywords

  • Human iPSC
  • Naive pluripotency
  • Neural differentiation
  • Reprogramming

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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    Kisa, F., Shiozawa, S., Oda, K., Yoshimatsu, S., Nakamura, M., Koya, I., Kawai, K., Suzuki, S., & Okano, H. (Accepted/In press). Naive-like ESRRB+ iPSCs with the Capacity for Rapid Neural Differentiation. Stem Cell Reports. https://doi.org/10.1016/j.stemcr.2017.10.008