NALT M cells are important for immune induction for the common mucosal immune system

Yasuhiro Date, Masashi Ebisawa, Shinji Fukuda, Hideaki Shima, Yuuki Obata, Daisuke Takahashi, Tamotsu Kato, Misaho Hanazato, Gaku Nakato, Ifor R. Williams, Kouji Hase, Hiroshi Ohno

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nasopharynx-associated lymphoid tissue (NALT) is one of the major constituents of the mucosaassociated lymphoid tissue (MALT), and has the ability to induce antigen-specific immune responses. However, the molecular mechanisms responsible for antigen uptake from the nasal cavity into the NALT remain largely unknown. Immunohistochemical analysis showed that CCL9 and CCL20 were co-localized with glycoprotein 2 (GP2) in the epithelium covering NALT, suggesting the existence of M cells in NALT. In analogy with the reduced number of Peyer's patch M cells in CCR6-deficient mice, the number of NALT M cells was drastically decreased in CCR6-deficient mice compared with the wild-type mice. Translocation of nasally administered Salmonella enterica serovar Typhimurium into NALT via NALT M cells was impaired in CCR6-deficient mice, whereas S. Typhimurium demonstrated consistent co-localization with NALT M cells in wild-type mice. When wild-type mice were nasally administered with an attenuated vaccine strain of S. Typhimurium, the mice were protected from a subsequent challenge with wild-type S. Typhimurium. Antigen-specific fecal and nasal IgA was detected after nasal immunization with the attenuated vaccine strain of S. Typhimurium only in wild-type mice but not in CCR6-deficient mice. Taken together, these observations demonstrate that NALT M cells are important as a first line of defense against infection by enabling activation of the common mucosal immune system (CMIS).

Original languageEnglish
Pages (from-to)471-478
Number of pages8
JournalInternational Immunology
Volume29
Issue number10
DOIs
Publication statusPublished - 2017 Oct 1

Fingerprint

Nasopharynx
Lymphoid Tissue
Immune System
Attenuated Vaccines
Nose
Antigens
Peyer's Patches
Salmonella enterica
Nasal Cavity
Histocompatibility Antigens Class II
Immunoglobulin A
Immunization
Glycoproteins
Epithelium

Keywords

  • CCR6
  • CMIS
  • GP2
  • Mucosal vaccination
  • Nasopharynx-associated lymphoid tissue

ASJC Scopus subject areas

  • Immunology

Cite this

NALT M cells are important for immune induction for the common mucosal immune system. / Date, Yasuhiro; Ebisawa, Masashi; Fukuda, Shinji; Shima, Hideaki; Obata, Yuuki; Takahashi, Daisuke; Kato, Tamotsu; Hanazato, Misaho; Nakato, Gaku; Williams, Ifor R.; Hase, Kouji; Ohno, Hiroshi.

In: International Immunology, Vol. 29, No. 10, 01.10.2017, p. 471-478.

Research output: Contribution to journalArticle

Date, Y, Ebisawa, M, Fukuda, S, Shima, H, Obata, Y, Takahashi, D, Kato, T, Hanazato, M, Nakato, G, Williams, IR, Hase, K & Ohno, H 2017, 'NALT M cells are important for immune induction for the common mucosal immune system', International Immunology, vol. 29, no. 10, pp. 471-478. https://doi.org/10.1093/intimm/dxx064
Date, Yasuhiro ; Ebisawa, Masashi ; Fukuda, Shinji ; Shima, Hideaki ; Obata, Yuuki ; Takahashi, Daisuke ; Kato, Tamotsu ; Hanazato, Misaho ; Nakato, Gaku ; Williams, Ifor R. ; Hase, Kouji ; Ohno, Hiroshi. / NALT M cells are important for immune induction for the common mucosal immune system. In: International Immunology. 2017 ; Vol. 29, No. 10. pp. 471-478.
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