Nano-scale physical surface coating of temperature-responsive polymers for cell sheet fabrication

Masamichi Nakayama, Yurika Kimura, Hideko Kanazawa, Masayuki Yamato, Teruo Okano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This study focused on the simple fabrication method of temperature-responsive cell culture surfaces by physical polymer coating. We synthesized diblock copolymers of poly(butyl methacrylate)-b-poly(N-isopropylacrylamide) (PBMA-b-PIPAAm) with various thermoresponsive PIPAAm chain length. Polymers were dissolved in mixed solvent of acetonitrile/N, N-dimethylformamide (5/1 in v/v), and the solution was deposited on the surface of commercial cell culture substrates using a spin coater. Chemically connected hydrophobic PBMA significantly enhanced physical absorption to target material surfaces and resulted in introducing thermoresponsive property to solid surfaces. To investigate temperature-dependent cellular behavior, bovine carotid artery endothelial cells were seeded on polymer-coated surfaces and cultured at 37 °C. Cells adhered and proliferated on temperature-responsive surface at 37 °C, while the efficiency of adhesion and proliferation became lower with increasing PIPAAm chain length. On the other hand, longer PIPAAm chains significantly promoted cell detachment from the surfaces by reducing temperature to 20 °C. Consequently, confluently cultured cells were successfully harvested as a single cell monolayer with maintaining cell-cell junctions and extracellular matrix proteins with simple low temperature treatment by choosing the adequate PIPAAm chain length of block copolymer.

Original languageEnglish
Title of host publication2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Print)9781479966790
DOIs
Publication statusPublished - 2015 Jan 9
Event2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014 - Nagoya, Japan
Duration: 2014 Nov 102014 Nov 12

Other

Other2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
CountryJapan
CityNagoya
Period14/11/1014/11/12

Fingerprint

Fabrication
Coatings
Polymers
Chain length
Temperature
Cell culture
Block copolymers
Endothelial cells
Dimethylformamide
Acetonitrile
Monolayers
Adhesion
Cells
Proteins
Substrates

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering

Cite this

Nakayama, M., Kimura, Y., Kanazawa, H., Yamato, M., & Okano, T. (2015). Nano-scale physical surface coating of temperature-responsive polymers for cell sheet fabrication. In 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014 [7006167] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MHS.2014.7006167

Nano-scale physical surface coating of temperature-responsive polymers for cell sheet fabrication. / Nakayama, Masamichi; Kimura, Yurika; Kanazawa, Hideko; Yamato, Masayuki; Okano, Teruo.

2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014. Institute of Electrical and Electronics Engineers Inc., 2015. 7006167.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nakayama, M, Kimura, Y, Kanazawa, H, Yamato, M & Okano, T 2015, Nano-scale physical surface coating of temperature-responsive polymers for cell sheet fabrication. in 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014., 7006167, Institute of Electrical and Electronics Engineers Inc., 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014, Nagoya, Japan, 14/11/10. https://doi.org/10.1109/MHS.2014.7006167
Nakayama M, Kimura Y, Kanazawa H, Yamato M, Okano T. Nano-scale physical surface coating of temperature-responsive polymers for cell sheet fabrication. In 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014. Institute of Electrical and Electronics Engineers Inc. 2015. 7006167 https://doi.org/10.1109/MHS.2014.7006167
Nakayama, Masamichi ; Kimura, Yurika ; Kanazawa, Hideko ; Yamato, Masayuki ; Okano, Teruo. / Nano-scale physical surface coating of temperature-responsive polymers for cell sheet fabrication. 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014. Institute of Electrical and Electronics Engineers Inc., 2015.
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