TY - JOUR
T1 - Nanodiamonds Inhibit Cancer Cell Migration by Strengthening Cell Adhesion
T2 - Implications for Cancer Treatment
AU - Guo, Qingyue
AU - Li, Lei
AU - Gao, Guanyue
AU - Liu, Runze
AU - Einaga, Yasuaki
AU - Zhi, Jinfang
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (no. 21874143 and no. 31770107), International Partnership Program of Chinese Academy of Sciences (grant no. GJHZ1818), and Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University.
Publisher Copyright:
©
PY - 2021/3/3
Y1 - 2021/3/3
N2 - Nanodiamonds (NDs) are a type of biocompatible nanomaterial with easily modified surfaces and are considered as promising candidates in biomedicine. In this work, the inhibition of tumor cell migration by carboxylated nanodiamonds (cNDs) was investigated. AFM-based single cell adhesion and F-actin staining experiments demonstrated that cNDs treatment could enhance cell adhesion and impair assembly of the cytoskeleton. The mechanism analysis of the regulatory protein expression level also proved that cNDs could inhibit the migration of Hela cells by preventing the epithelial-mesenchymal transition (EMT) process through the transforming growth factor β (TGF-β) signaling pathway. The in vivo pulmonary metastasis model also showed that cNDs effectively reduced the metastasis of murine B16 melanoma cells. In summary, cNDs have been demonstrated to inhibit cancer cell migration in vitro and decrease tumor metastasis in vivo. Therefore, cNDs might have potential utility for specific cancer treatment.
AB - Nanodiamonds (NDs) are a type of biocompatible nanomaterial with easily modified surfaces and are considered as promising candidates in biomedicine. In this work, the inhibition of tumor cell migration by carboxylated nanodiamonds (cNDs) was investigated. AFM-based single cell adhesion and F-actin staining experiments demonstrated that cNDs treatment could enhance cell adhesion and impair assembly of the cytoskeleton. The mechanism analysis of the regulatory protein expression level also proved that cNDs could inhibit the migration of Hela cells by preventing the epithelial-mesenchymal transition (EMT) process through the transforming growth factor β (TGF-β) signaling pathway. The in vivo pulmonary metastasis model also showed that cNDs effectively reduced the metastasis of murine B16 melanoma cells. In summary, cNDs have been demonstrated to inhibit cancer cell migration in vitro and decrease tumor metastasis in vivo. Therefore, cNDs might have potential utility for specific cancer treatment.
KW - atomic force microscopy
KW - carboxylated nanodiamond
KW - cell migration
KW - epithelial-mesenchymal transition
KW - patterned silicon membrane
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U2 - 10.1021/acsami.0c21332
DO - 10.1021/acsami.0c21332
M3 - Article
C2 - 33595291
AN - SCOPUS:85102479482
SN - 1944-8244
VL - 13
SP - 9620
EP - 9629
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 8
ER -
