Nanodiscs for structural biology in a membranous environment

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

The structures of many membrane proteins have been analyzed in detergent micelles. However, the environment of detergent micelles differs somewhat from that of the lipid bilayer, where membrane proteins exhibit physiological functions. Therefore, a more membrane-like environment has been awaited for structural analysis of membrane proteins. Nanodiscs are “hockey-puck”-shaped lipid bilayer particles that distribute in a monodispersed manner in aqueous solution. We review how nanodiscs or protein-reconstituted nanodiscs are prepared and how they are utilized to analyze protein structure, dynamics, and interactions with lipid molecules using solution NMR and cryo-electron microscopy.

Original languageEnglish
Pages (from-to)321-326
Number of pages6
JournalChemical and Pharmaceutical Bulletin
Volume67
Issue number4
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Cryo-electron microscopy
  • Functional analysis
  • Membrane protein
  • Nanodisc
  • Solution NMR
  • Structural analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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