Nanoimprinted holes to immobilize microbes

T. Kano, T. Inaba, N. Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper we demonstrate highly dense immobilization of bacteria into nanoimprinted holes. Nanoimprinting enables micro holes smaller than 2 μm in diameter with a high accuracy, which cannot be patterned using conventional UV photolithography. In our prior work, we developed a microbial reactor immobilizing bacteria into micro holes, which facilitated collection and evaluation of reaction products while the number of bacteria involved in the reaction could be quantified. However, the holes were made by photolithography and the minimum size was limited to be 3 μm in diameter. Large holes allow multiple bacteria to be immobilized in a hole, which resulted in errors in quantification. The number of bacteria immobilized in a nanoimprinted hole was found to have smaller deviation than in photolithographically formed holes. In addition, density of the immobilized bacteria was experimentally found to be largest in case of 2-μm-holes. The proposed processes will be of great help for precise evaluation of bacteria reaction.

Original languageEnglish
Title of host publicationIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages963-964
Number of pages2
DOIs
Publication statusPublished - 2013 Apr 2
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Kano, T., Inaba, T., & Miki, N. (2013). Nanoimprinted holes to immobilize microbes. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 963-964). [6474406] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474406