Nanoimprinted holes to immobilize microbes

T. Kano, T. Inaba, Norihisa Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper we demonstrate highly dense immobilization of bacteria into nanoimprinted holes. Nanoimprinting enables micro holes smaller than 2 μm in diameter with a high accuracy, which cannot be patterned using conventional UV photolithography. In our prior work, we developed a microbial reactor immobilizing bacteria into micro holes, which facilitated collection and evaluation of reaction products while the number of bacteria involved in the reaction could be quantified. However, the holes were made by photolithography and the minimum size was limited to be 3 μm in diameter. Large holes allow multiple bacteria to be immobilized in a hole, which resulted in errors in quantification. The number of bacteria immobilized in a nanoimprinted hole was found to have smaller deviation than in photolithographically formed holes. In addition, density of the immobilized bacteria was experimentally found to be largest in case of 2-μm-holes. The proposed processes will be of great help for precise evaluation of bacteria reaction.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages963-964
Number of pages2
DOIs
Publication statusPublished - 2013
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

Fingerprint

microorganisms
Bacteria
bacteria
Photolithography
photolithography
Reaction products
evaluation
immobilization
reaction products
reactors
deviation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Kano, T., Inaba, T., & Miki, N. (2013). Nanoimprinted holes to immobilize microbes. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 963-964). [6474406] https://doi.org/10.1109/MEMSYS.2013.6474406

Nanoimprinted holes to immobilize microbes. / Kano, T.; Inaba, T.; Miki, Norihisa.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 963-964 6474406.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kano, T, Inaba, T & Miki, N 2013, Nanoimprinted holes to immobilize microbes. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6474406, pp. 963-964, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 13/1/20. https://doi.org/10.1109/MEMSYS.2013.6474406
Kano T, Inaba T, Miki N. Nanoimprinted holes to immobilize microbes. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 963-964. 6474406 https://doi.org/10.1109/MEMSYS.2013.6474406
Kano, T. ; Inaba, T. ; Miki, Norihisa. / Nanoimprinted holes to immobilize microbes. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. pp. 963-964
@inproceedings{62794496539c4a13b9c3f48622919e58,
title = "Nanoimprinted holes to immobilize microbes",
abstract = "In this paper we demonstrate highly dense immobilization of bacteria into nanoimprinted holes. Nanoimprinting enables micro holes smaller than 2 μm in diameter with a high accuracy, which cannot be patterned using conventional UV photolithography. In our prior work, we developed a microbial reactor immobilizing bacteria into micro holes, which facilitated collection and evaluation of reaction products while the number of bacteria involved in the reaction could be quantified. However, the holes were made by photolithography and the minimum size was limited to be 3 μm in diameter. Large holes allow multiple bacteria to be immobilized in a hole, which resulted in errors in quantification. The number of bacteria immobilized in a nanoimprinted hole was found to have smaller deviation than in photolithographically formed holes. In addition, density of the immobilized bacteria was experimentally found to be largest in case of 2-μm-holes. The proposed processes will be of great help for precise evaluation of bacteria reaction.",
author = "T. Kano and T. Inaba and Norihisa Miki",
year = "2013",
doi = "10.1109/MEMSYS.2013.6474406",
language = "English",
isbn = "9781467356558",
pages = "963--964",
booktitle = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

}

TY - GEN

T1 - Nanoimprinted holes to immobilize microbes

AU - Kano, T.

AU - Inaba, T.

AU - Miki, Norihisa

PY - 2013

Y1 - 2013

N2 - In this paper we demonstrate highly dense immobilization of bacteria into nanoimprinted holes. Nanoimprinting enables micro holes smaller than 2 μm in diameter with a high accuracy, which cannot be patterned using conventional UV photolithography. In our prior work, we developed a microbial reactor immobilizing bacteria into micro holes, which facilitated collection and evaluation of reaction products while the number of bacteria involved in the reaction could be quantified. However, the holes were made by photolithography and the minimum size was limited to be 3 μm in diameter. Large holes allow multiple bacteria to be immobilized in a hole, which resulted in errors in quantification. The number of bacteria immobilized in a nanoimprinted hole was found to have smaller deviation than in photolithographically formed holes. In addition, density of the immobilized bacteria was experimentally found to be largest in case of 2-μm-holes. The proposed processes will be of great help for precise evaluation of bacteria reaction.

AB - In this paper we demonstrate highly dense immobilization of bacteria into nanoimprinted holes. Nanoimprinting enables micro holes smaller than 2 μm in diameter with a high accuracy, which cannot be patterned using conventional UV photolithography. In our prior work, we developed a microbial reactor immobilizing bacteria into micro holes, which facilitated collection and evaluation of reaction products while the number of bacteria involved in the reaction could be quantified. However, the holes were made by photolithography and the minimum size was limited to be 3 μm in diameter. Large holes allow multiple bacteria to be immobilized in a hole, which resulted in errors in quantification. The number of bacteria immobilized in a nanoimprinted hole was found to have smaller deviation than in photolithographically formed holes. In addition, density of the immobilized bacteria was experimentally found to be largest in case of 2-μm-holes. The proposed processes will be of great help for precise evaluation of bacteria reaction.

UR - http://www.scopus.com/inward/record.url?scp=84875470193&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875470193&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2013.6474406

DO - 10.1109/MEMSYS.2013.6474406

M3 - Conference contribution

SN - 9781467356558

SP - 963

EP - 964

BT - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

ER -