TY - JOUR
T1 - Nanopore sequencing
T2 - Review of potential applications in functional genomics
AU - Kono, Nobuaki
AU - Arakawa, Kazuharu
N1 - Funding Information:
ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan); Yamagata Prefectural Government and Tsuruoka City, Japan
Funding Information:
The authors would like to thank Dr. James Fleming for his diligent proofreading of this manuscript. This work was funded by the ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan) and in part by research funds from the Yamagata Prefectural Government and Tsuruoka City, Japan.
Publisher Copyright:
© 2019 Japanese Society of Developmental Biologists
PY - 2019/6
Y1 - 2019/6
N2 - Molecular biology has been led by various measurement technologies, and increased throughput has developed omics analysis. The development of massively parallel sequencing technology has enabled access to fundamental molecular data and revealed genomic and transcriptomic signatures. Nanopore sequencers have driven such evolution to the next stage. Oxford Nanopore Technologies Inc. provides a new type of single molecule sequencer using protein nanopore that realizes direct sequencing without DNA synthesizing or amplification. This nanopore sequencer can sequence an ultra-long read limited by the input nucleotide length, or can determine DNA/RNA modifications. Recently, many fields such as medicine, epidemiology, ecology, and education have benefited from this technology. In this review, we explain the features and functions of the nanopore sequencer, introduce various situations where it has been used as a critical technology, and expected future applications.
AB - Molecular biology has been led by various measurement technologies, and increased throughput has developed omics analysis. The development of massively parallel sequencing technology has enabled access to fundamental molecular data and revealed genomic and transcriptomic signatures. Nanopore sequencers have driven such evolution to the next stage. Oxford Nanopore Technologies Inc. provides a new type of single molecule sequencer using protein nanopore that realizes direct sequencing without DNA synthesizing or amplification. This nanopore sequencer can sequence an ultra-long read limited by the input nucleotide length, or can determine DNA/RNA modifications. Recently, many fields such as medicine, epidemiology, ecology, and education have benefited from this technology. In this review, we explain the features and functions of the nanopore sequencer, introduce various situations where it has been used as a critical technology, and expected future applications.
KW - long reads
KW - nanopore sequencing
KW - next generation sequencing
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U2 - 10.1111/dgd.12608
DO - 10.1111/dgd.12608
M3 - Review article
C2 - 31037722
AN - SCOPUS:85065168734
SN - 0012-1592
VL - 61
SP - 316
EP - 326
JO - Development, growth & differentiation
JF - Development, growth & differentiation
IS - 5
ER -