Na+-driven multidrug efflux pump VcmA from Vibrio cholerae non-O1, a non-halophilic bacterium

M. Nazmul Huda, Yuji Morita, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A chromosomal DNA fragment from Vibrio cholerae non-O1 containing a drug resistance determinant was cloned and sequenced. The deduced amino acid sequence suggested that the determinant gene encodes a multidrug efflux pump. We designated the pump VcmA. Escherichia coli cells transformed with a plasmid carrying the vcmA gene showed increased resistance against norfloxacin, ciprofloxacin, ofloxacin, daunomycin, doxorubicin, streptomycin, kanamycin, ethidium bromide, 4′,6-diamidino-2-phenylindole dihydrochloride, Hoechst 33342 and acriflavine. Na+ (or Li+)-dependent efflux of ethidium bromide was detected in transformant cells. Efflux of Na+, elicited by ethidium bromide, was observed from transformant cells. Thus, we concluded that the VcmA is a Na+/drug antiporter.

Original languageEnglish
Pages (from-to)235-239
Number of pages5
JournalFEMS Microbiology Letters
Volume203
Issue number2
DOIs
Publication statusPublished - 2001 Sep 25
Externally publishedYes

Fingerprint

Vibrio cholerae non-O1
Ethidium
Bacteria
Acriflavine
Antiporters
Norfloxacin
Daunorubicin
Kanamycin
Ofloxacin
Streptomycin
Ciprofloxacin
Drug Resistance
Doxorubicin
Genes
Amino Acid Sequence
Plasmids
Escherichia coli
DNA
Pharmaceutical Preparations

Keywords

  • Drug resistance
  • Na/drug antiporter
  • VcmA drug efflux pump
  • Vibrio cholerae

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Na+-driven multidrug efflux pump VcmA from Vibrio cholerae non-O1, a non-halophilic bacterium. / Huda, M. Nazmul; Morita, Yuji; Kuroda, Teruo; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: FEMS Microbiology Letters, Vol. 203, No. 2, 25.09.2001, p. 235-239.

Research output: Contribution to journalArticle

Huda, M. Nazmul ; Morita, Yuji ; Kuroda, Teruo ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / Na+-driven multidrug efflux pump VcmA from Vibrio cholerae non-O1, a non-halophilic bacterium. In: FEMS Microbiology Letters. 2001 ; Vol. 203, No. 2. pp. 235-239.
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