TY - JOUR
T1 - Na+, K+-ATPase inhibitory activity of fractionated urine during changes in dietary sodium intake in man
AU - Imafuku, T.
AU - Ogawa, Z.
AU - Itoh, H.
AU - Suzuki, H.
AU - Saruta, T.
PY - 1990
Y1 - 1990
N2 - Na+-, K+-ATPase inhibitory activity in urine fractionated by HPLC was quantified in 7 normotensive male subjects during changes in dietary sodium intake. Subjects were studied on free sodium intake for 2 days, on low sodium intake (2 g/day) for 3 days, on high sodium intake (22 g/day) for 4 days and subsequently on normal sodium intake (6 g/day) for 2 days. Na+, K+-ATPase inhibitory activity in fraction 10 eluted with 17% acetonitrile by reverse-phase HPLC was 12.3 ± 5.2% (mean ± S.D.) on free sodium intake, 8.7 ± 9.8% on the 3rd day of low sodium intake, 61.2 ± 6.6% on the 4th day of high sodium intake, and 20.5% ± 0.7% on the 2nd day of the normal sodium intake. Changes in Na+, K+-ATPase inhibitory activity of fraction 10 were closely associated with those in urinary sodium excretion. These results suggest that an endogenous Na+, K+-ATPase inhibitor(s) which plays a physiological role in the control of sodium and water balance may exist in this particular fraction.
AB - Na+-, K+-ATPase inhibitory activity in urine fractionated by HPLC was quantified in 7 normotensive male subjects during changes in dietary sodium intake. Subjects were studied on free sodium intake for 2 days, on low sodium intake (2 g/day) for 3 days, on high sodium intake (22 g/day) for 4 days and subsequently on normal sodium intake (6 g/day) for 2 days. Na+, K+-ATPase inhibitory activity in fraction 10 eluted with 17% acetonitrile by reverse-phase HPLC was 12.3 ± 5.2% (mean ± S.D.) on free sodium intake, 8.7 ± 9.8% on the 3rd day of low sodium intake, 61.2 ± 6.6% on the 4th day of high sodium intake, and 20.5% ± 0.7% on the 2nd day of the normal sodium intake. Changes in Na+, K+-ATPase inhibitory activity of fraction 10 were closely associated with those in urinary sodium excretion. These results suggest that an endogenous Na+, K+-ATPase inhibitor(s) which plays a physiological role in the control of sodium and water balance may exist in this particular fraction.
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U2 - 10.1507/endocrj1954.37.39
DO - 10.1507/endocrj1954.37.39
M3 - Article
C2 - 2143468
AN - SCOPUS:0025289551
SN - 0918-8959
VL - 37
SP - 39
EP - 46
JO - Endocrine Journal
JF - Endocrine Journal
IS - 1
ER -