Na+/Mg2+ transporter acts as a Mg2+ buffering mechanism in PC12 cells

Takeshi Kubota, Kentaro Tokuno, Jun Nakagawa, Yoshiichiro Kitamura, Hiroto Ogawa, Yoshio Suzuki, Koji Suzuki, Kotaro Oka

Research output: Contribution to journalArticle

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Abstract

Mg2+ buffering mechanisms in PC12 cells were demonstrated with particular focus on the role of the Na+/Mg2+ transporter by using a newly developed Mg2+ indicator, KMG-20, and also a Na+ indicator, Sodium Green. Carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP), a protonophore, induced a transient increase in the intracellular Mg2+ concentration ([Mg2+]i). The rate of decrease of [Mg2+]i was slower in a Na+-free extracellular medium, suggesting the coupling of Na+ influx and Mg2+ efflux. Na+ influxes were different for normal and imipramine- (a putative inhibitor of the Na+/Mg2+ transporter) containing solutions. FCCP induced a rapid increase in [Na+]i in the normal solution, while the increase was gradual in the imipramine-containing solution. The rate of decrease of [Mg2+]i in the imipramine-containing solution was also slower than that in the normal solution. From these results, we show that the main buffering mechanism for excess Mg2+ depends on the Na+/Mg2+ transporter in PC12 cells.

Original languageEnglish
Pages (from-to)332-336
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume303
Issue number1
DOIs
Publication statusPublished - 2003 Mar 28

Fingerprint

PC12 Cells
Imipramine
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone

Keywords

  • FCCP
  • Imipramine
  • KMG-20
  • Sodium Green

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Na+/Mg2+ transporter acts as a Mg2+ buffering mechanism in PC12 cells. / Kubota, Takeshi; Tokuno, Kentaro; Nakagawa, Jun; Kitamura, Yoshiichiro; Ogawa, Hiroto; Suzuki, Yoshio; Suzuki, Koji; Oka, Kotaro.

In: Biochemical and Biophysical Research Communications, Vol. 303, No. 1, 28.03.2003, p. 332-336.

Research output: Contribution to journalArticle

Kubota, T, Tokuno, K, Nakagawa, J, Kitamura, Y, Ogawa, H, Suzuki, Y, Suzuki, K & Oka, K 2003, 'Na+/Mg2+ transporter acts as a Mg2+ buffering mechanism in PC12 cells', Biochemical and Biophysical Research Communications, vol. 303, no. 1, pp. 332-336. https://doi.org/10.1016/S0006-291X(03)00346-2
Kubota, Takeshi ; Tokuno, Kentaro ; Nakagawa, Jun ; Kitamura, Yoshiichiro ; Ogawa, Hiroto ; Suzuki, Yoshio ; Suzuki, Koji ; Oka, Kotaro. / Na+/Mg2+ transporter acts as a Mg2+ buffering mechanism in PC12 cells. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 303, No. 1. pp. 332-336.
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