Nationwide surveillance of antimicrobial susceptibility patterns of pathogens isolated from surgical site infections (SSI) in Japan

Yoshio Takesue, Akira Watanabe, Hideaki Hanaki, Shinya Kusachi, Tetsuro Matsumoto, Aikichi Iwamoto, Kyoichi Totsuka, Keisuke Sunakawa, Morimasa Yagisawa, Junko Sato, Toyoko Oguri, Kunio Nakanishi, Yoshinobu Sumiyama, Yuukou Kitagawa, Go Wakabayashi, Isamu Koyama, Katsuhiko Yanaga, Toshiro Konishi, Ryoji Fukushima, Shiko Seki & 15 others Shun Imai, Tsunehiro Shintani, Hiroki Tsukada, Kazuhiro Tsukada, Kenji Omura, Hiroshige Mikamo, Hiromitsu Takeyama, Masato Kusunoki, Shoji Kubo, Junzo Shimizu, Toshihiro Hirai, Hiroki Ohge, Akio Kadowaki, Kohji Okamoto, Katsunori Yanagihara

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

To investigate the trends of antimicrobial resistance in pathogens isolated from surgical site infections (SSI), a Japanese surveillance committee conducted the first nationwide survey. Seven main organisms were collected from SSI at 27 medical centers in 2010 and were shipped to a central laboratory for antimicrobial susceptibility testing. A total of 702 isolates from 586 patients with SSI were included. Staphylococcus aureus (20.4 %) and Enterococcus faecalis (19.5 %) were the most common isolates, followed by Pseudomonas aeruginosa (15.4 %) and Bacteroides fragilis group (15.4 %). Methicillin-resistant S. aureus among S. aureus was 72.0 %. Vancomycin MIC 2 μg/ml strains accounted for 9.7 %. In Escherichia coli, 11 of 95 strains produced extended-spectrum β-lactamase (Klebsiella pneumoniae, 0/53 strains). Of E. coli strains, 8.4 % were resistant to ceftazidime (CAZ) and 26.3 % to ciprofloxacin (CPFX). No P. aeruginosa strains produced metallo-β-lactamase. In P. aeruginosa, the resistance rates were 7.4 % to tazobactam/piperacillin (TAZ/PIPC), 10.2 % to imipenem (IPM), 2.8 % to meropenem, cefepime, and CPFX, and 0 % to gentamicin. In the B. fragilis group, the rates were 28.6 % to clindamycin, 5.7 % to cefmetazole, 2.9 % to TAZ/PIPC and IPM, and 0 % to metronidazole (Bacteroides thetaiotaomicron; 59.1, 36.4, 0, 0, 0 %). MIC90 of P. aeruginosa isolated 15 days or later after surgery rose in TAZ/PIPC, CAZ, IPM, and CPFX. In patients with American Society of Anesthesiologists (ASA) score ≥3, the resistance rates of P. aeruginosa to TAZ/PIPC and CAZ were higher than in patients with ASA ≤2. The data obtained in this study revealed the trend of the spread of resistance among common species that cause SSI. Timing of isolation from surgery and the patient's physical status affected the selection of resistant organisms.

Original languageEnglish
Pages (from-to)816-826
Number of pages11
JournalJournal of Infection and Chemotherapy
Volume18
Issue number6
DOIs
Publication statusPublished - 2012 Dec

Fingerprint

Surgical Wound Infection
Pseudomonas aeruginosa
Japan
Ceftazidime
Imipenem
Ciprofloxacin
Bacteroides fragilis
meropenem
Staphylococcus aureus
Cefmetazole
Patient Isolation
Escherichia coli
Clindamycin
Enterococcus faecalis
Metronidazole
Klebsiella pneumoniae
Vancomycin
Methicillin-Resistant Staphylococcus aureus
Gentamicins
tazobactam drug combination piperacillin

Keywords

  • Antibiotic susceptibility
  • Bacteroides fragilis group
  • Surgical site infections
  • Surveillance

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases
  • Medicine(all)

Cite this

Nationwide surveillance of antimicrobial susceptibility patterns of pathogens isolated from surgical site infections (SSI) in Japan. / Takesue, Yoshio; Watanabe, Akira; Hanaki, Hideaki; Kusachi, Shinya; Matsumoto, Tetsuro; Iwamoto, Aikichi; Totsuka, Kyoichi; Sunakawa, Keisuke; Yagisawa, Morimasa; Sato, Junko; Oguri, Toyoko; Nakanishi, Kunio; Sumiyama, Yoshinobu; Kitagawa, Yuukou; Wakabayashi, Go; Koyama, Isamu; Yanaga, Katsuhiko; Konishi, Toshiro; Fukushima, Ryoji; Seki, Shiko; Imai, Shun; Shintani, Tsunehiro; Tsukada, Hiroki; Tsukada, Kazuhiro; Omura, Kenji; Mikamo, Hiroshige; Takeyama, Hiromitsu; Kusunoki, Masato; Kubo, Shoji; Shimizu, Junzo; Hirai, Toshihiro; Ohge, Hiroki; Kadowaki, Akio; Okamoto, Kohji; Yanagihara, Katsunori.

In: Journal of Infection and Chemotherapy, Vol. 18, No. 6, 12.2012, p. 816-826.

Research output: Contribution to journalArticle

Takesue, Y, Watanabe, A, Hanaki, H, Kusachi, S, Matsumoto, T, Iwamoto, A, Totsuka, K, Sunakawa, K, Yagisawa, M, Sato, J, Oguri, T, Nakanishi, K, Sumiyama, Y, Kitagawa, Y, Wakabayashi, G, Koyama, I, Yanaga, K, Konishi, T, Fukushima, R, Seki, S, Imai, S, Shintani, T, Tsukada, H, Tsukada, K, Omura, K, Mikamo, H, Takeyama, H, Kusunoki, M, Kubo, S, Shimizu, J, Hirai, T, Ohge, H, Kadowaki, A, Okamoto, K & Yanagihara, K 2012, 'Nationwide surveillance of antimicrobial susceptibility patterns of pathogens isolated from surgical site infections (SSI) in Japan', Journal of Infection and Chemotherapy, vol. 18, no. 6, pp. 816-826. https://doi.org/10.1007/s10156-012-0509-1
Takesue, Yoshio ; Watanabe, Akira ; Hanaki, Hideaki ; Kusachi, Shinya ; Matsumoto, Tetsuro ; Iwamoto, Aikichi ; Totsuka, Kyoichi ; Sunakawa, Keisuke ; Yagisawa, Morimasa ; Sato, Junko ; Oguri, Toyoko ; Nakanishi, Kunio ; Sumiyama, Yoshinobu ; Kitagawa, Yuukou ; Wakabayashi, Go ; Koyama, Isamu ; Yanaga, Katsuhiko ; Konishi, Toshiro ; Fukushima, Ryoji ; Seki, Shiko ; Imai, Shun ; Shintani, Tsunehiro ; Tsukada, Hiroki ; Tsukada, Kazuhiro ; Omura, Kenji ; Mikamo, Hiroshige ; Takeyama, Hiromitsu ; Kusunoki, Masato ; Kubo, Shoji ; Shimizu, Junzo ; Hirai, Toshihiro ; Ohge, Hiroki ; Kadowaki, Akio ; Okamoto, Kohji ; Yanagihara, Katsunori. / Nationwide surveillance of antimicrobial susceptibility patterns of pathogens isolated from surgical site infections (SSI) in Japan. In: Journal of Infection and Chemotherapy. 2012 ; Vol. 18, No. 6. pp. 816-826.
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AU - Takesue, Yoshio

AU - Watanabe, Akira

AU - Hanaki, Hideaki

AU - Kusachi, Shinya

AU - Matsumoto, Tetsuro

AU - Iwamoto, Aikichi

AU - Totsuka, Kyoichi

AU - Sunakawa, Keisuke

AU - Yagisawa, Morimasa

AU - Sato, Junko

AU - Oguri, Toyoko

AU - Nakanishi, Kunio

AU - Sumiyama, Yoshinobu

AU - Kitagawa, Yuukou

AU - Wakabayashi, Go

AU - Koyama, Isamu

AU - Yanaga, Katsuhiko

AU - Konishi, Toshiro

AU - Fukushima, Ryoji

AU - Seki, Shiko

AU - Imai, Shun

AU - Shintani, Tsunehiro

AU - Tsukada, Hiroki

AU - Tsukada, Kazuhiro

AU - Omura, Kenji

AU - Mikamo, Hiroshige

AU - Takeyama, Hiromitsu

AU - Kusunoki, Masato

AU - Kubo, Shoji

AU - Shimizu, Junzo

AU - Hirai, Toshihiro

AU - Ohge, Hiroki

AU - Kadowaki, Akio

AU - Okamoto, Kohji

AU - Yanagihara, Katsunori

PY - 2012/12

Y1 - 2012/12

N2 - To investigate the trends of antimicrobial resistance in pathogens isolated from surgical site infections (SSI), a Japanese surveillance committee conducted the first nationwide survey. Seven main organisms were collected from SSI at 27 medical centers in 2010 and were shipped to a central laboratory for antimicrobial susceptibility testing. A total of 702 isolates from 586 patients with SSI were included. Staphylococcus aureus (20.4 %) and Enterococcus faecalis (19.5 %) were the most common isolates, followed by Pseudomonas aeruginosa (15.4 %) and Bacteroides fragilis group (15.4 %). Methicillin-resistant S. aureus among S. aureus was 72.0 %. Vancomycin MIC 2 μg/ml strains accounted for 9.7 %. In Escherichia coli, 11 of 95 strains produced extended-spectrum β-lactamase (Klebsiella pneumoniae, 0/53 strains). Of E. coli strains, 8.4 % were resistant to ceftazidime (CAZ) and 26.3 % to ciprofloxacin (CPFX). No P. aeruginosa strains produced metallo-β-lactamase. In P. aeruginosa, the resistance rates were 7.4 % to tazobactam/piperacillin (TAZ/PIPC), 10.2 % to imipenem (IPM), 2.8 % to meropenem, cefepime, and CPFX, and 0 % to gentamicin. In the B. fragilis group, the rates were 28.6 % to clindamycin, 5.7 % to cefmetazole, 2.9 % to TAZ/PIPC and IPM, and 0 % to metronidazole (Bacteroides thetaiotaomicron; 59.1, 36.4, 0, 0, 0 %). MIC90 of P. aeruginosa isolated 15 days or later after surgery rose in TAZ/PIPC, CAZ, IPM, and CPFX. In patients with American Society of Anesthesiologists (ASA) score ≥3, the resistance rates of P. aeruginosa to TAZ/PIPC and CAZ were higher than in patients with ASA ≤2. The data obtained in this study revealed the trend of the spread of resistance among common species that cause SSI. Timing of isolation from surgery and the patient's physical status affected the selection of resistant organisms.

AB - To investigate the trends of antimicrobial resistance in pathogens isolated from surgical site infections (SSI), a Japanese surveillance committee conducted the first nationwide survey. Seven main organisms were collected from SSI at 27 medical centers in 2010 and were shipped to a central laboratory for antimicrobial susceptibility testing. A total of 702 isolates from 586 patients with SSI were included. Staphylococcus aureus (20.4 %) and Enterococcus faecalis (19.5 %) were the most common isolates, followed by Pseudomonas aeruginosa (15.4 %) and Bacteroides fragilis group (15.4 %). Methicillin-resistant S. aureus among S. aureus was 72.0 %. Vancomycin MIC 2 μg/ml strains accounted for 9.7 %. In Escherichia coli, 11 of 95 strains produced extended-spectrum β-lactamase (Klebsiella pneumoniae, 0/53 strains). Of E. coli strains, 8.4 % were resistant to ceftazidime (CAZ) and 26.3 % to ciprofloxacin (CPFX). No P. aeruginosa strains produced metallo-β-lactamase. In P. aeruginosa, the resistance rates were 7.4 % to tazobactam/piperacillin (TAZ/PIPC), 10.2 % to imipenem (IPM), 2.8 % to meropenem, cefepime, and CPFX, and 0 % to gentamicin. In the B. fragilis group, the rates were 28.6 % to clindamycin, 5.7 % to cefmetazole, 2.9 % to TAZ/PIPC and IPM, and 0 % to metronidazole (Bacteroides thetaiotaomicron; 59.1, 36.4, 0, 0, 0 %). MIC90 of P. aeruginosa isolated 15 days or later after surgery rose in TAZ/PIPC, CAZ, IPM, and CPFX. In patients with American Society of Anesthesiologists (ASA) score ≥3, the resistance rates of P. aeruginosa to TAZ/PIPC and CAZ were higher than in patients with ASA ≤2. The data obtained in this study revealed the trend of the spread of resistance among common species that cause SSI. Timing of isolation from surgery and the patient's physical status affected the selection of resistant organisms.

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KW - Bacteroides fragilis group

KW - Surgical site infections

KW - Surveillance

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