Natriuretic peptides in developing medaka embryos: Implications in cardiac development by loss-of-function studies

Hiroshi Miyanishi, Kataaki Okubo, Shigenori Nobata, Yoshio Takei

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cardiac natriuretic peptides (NPs), atrial NP (ANP) and B-type NP (BNP), and their receptor, guanylyl cyclase (GC)-A have attracted attention of many basic and clinical researchers because of their potent renal and cardiovascular actions. In this study, we used medaka, Oryzias latipes, as a model species to pursue the physiological functions of NPs because it is a suitable model for developmental analyses. Medaka has two ligands, BNP and C-type NP3 (CNP3) (but not ANP), that have greater affinity for thetwo O. latipes GC-A receptors (OLGC), OLGC7 and OLGC2, respectively. CNP3 is the ancestral molecule of cardiac NPs. Initially, we examined developmental expression of cardiac NP/receptor combinations, BNP/OLGC7 and CNP3/OLGC2, using quantitative real-time PCR and in situ hybridization. BNP and CNP3 mRNA increased at stages 25 (onset of ventricular formation) and 22 (appearance of heart anlage), respectively, whereas both receptor mRNAs increased at as early as stage 12. BNP/OLGC7 transcripts were found in arterial/ventricular tissues and CNP3/OLGC2 transcripts in venous/atrial tissues by in situ hybridization. Thus, BNP and CNP3 can act locally on cardiac myocytes in a paracrine/autocrine fashion. Double knockdown of BNP/OLGC7 genes impaired ventricular development by causing hypoplasia of ventricular myocytes as evidenced by reduced bromodeoxyuridine incorporation. CNP3 knockdown induced hypertrophy of atria and activated the renin-angiotensin system. Collectively, it appears that BNP is important for normal ventricular, whereas CNP3 is important for normal atrial development and performance, a role usually taken by ANP in other vertebrates. The current study provides new insights into the role of cardiac NPs in cardiac development in vertebrates.

Original languageEnglish
Pages (from-to)410-420
Number of pages11
JournalEndocrinology
Volume154
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

Fingerprint

Oryzias
Natriuretic Peptides
Embryonic Structures
In Situ Hybridization
Vertebrates
Messenger RNA
Brain Natriuretic Peptide
Atrial Natriuretic Factor
Bromodeoxyuridine
Renin-Angiotensin System
Cardiac Myocytes
Muscle Cells
Hypertrophy
Real-Time Polymerase Chain Reaction
Research Personnel
Ligands
Kidney
Genes
atrial natriuretic factor receptor B
atrial natriuretic factor receptor A

ASJC Scopus subject areas

  • Endocrinology

Cite this

Natriuretic peptides in developing medaka embryos : Implications in cardiac development by loss-of-function studies. / Miyanishi, Hiroshi; Okubo, Kataaki; Nobata, Shigenori; Takei, Yoshio.

In: Endocrinology, Vol. 154, No. 1, 01.01.2013, p. 410-420.

Research output: Contribution to journalArticle

Miyanishi, Hiroshi ; Okubo, Kataaki ; Nobata, Shigenori ; Takei, Yoshio. / Natriuretic peptides in developing medaka embryos : Implications in cardiac development by loss-of-function studies. In: Endocrinology. 2013 ; Vol. 154, No. 1. pp. 410-420.
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