Natriuretic peptides/cGMP/cGMP-dependent protein kinase cascades promote muscle mitochondrial biogenesis and prevent obesity

Kazutoshi Miyashita, Hiroshi Itoh, Hirokazu Tsujimoto, Naohisa Tamura, Yasutomo Fukunaga, Masakatsu Sone, Kenichi Yamahara, Daisuke Taura, Megumi Inuzuka, Takuhiro Sonoyama, Kazuwa Nakao

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE - Natriuretic peptides (NPs) have been characterized as vascular hormones that regulate vascular tone via guanylyl cyclase (GC), cyclic GMP (cGMP), and cGMP-dependent protein kinase (cGK). Recent clinical studies have shown that plasma NP levels were lower in subjects with the metabolic syndrome. The present study was conducted to elucidate the roles for NP/cGK cascades in energy metabolism. RESEARCH DESIGN AND METHODS-We used three types of genetically engineered mice: brain NP (BNP) transgenic (BNP-Tg), cGK-Tg, and guanylyl cyclase-A (GCA) heterozygous knockout (GCA+/-) mice and analyzed the metabolic consequences of chronic activation of NP/cGK cascades in vivo. We also examined the effect of NPs in cultured myocytes. RESULTS - BNP-Tg mice fed on high-fat diet were protected against diet-induced obesity and insulin resistance, and cGK-Tg mice had reduced body weight even on standard diet; surprisingly, giant mitochondria were densely packed in the skeletal muscle. Both mice showed an increase in muscle mitochondrial content and fat oxidation through upregulation of peroxisome proliferator-activated receptor (PPAR)-γ coactivator (PGC)-1α and PPARδ. The functional NP receptors, GCA and guanylyl cyclase-B, were downregulated by feeding a high-fat diet, while GCA+/- mice showed increases in body weight and glucose intolerance when fed a high-fat diet. NPs directly increased the expression of PGC-1α and PPARδ and mitochondrial content in cultured myocytes. CONCLUSIONS - The findings together suggest that NP/cGK cascades can promote muscle mitochondrial biogenesis and fat oxidation, as to prevent obesity and glucose intolerance. The vascular hormone, NP, would contribute to coordinated regulation of oxygen supply and consumption.

Original languageEnglish
Pages (from-to)2880-2892
Number of pages13
JournalDiabetes
Volume58
Issue number12
DOIs
Publication statusPublished - 2009 Dec

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Cyclic GMP-Dependent Protein Kinases
Natriuretic Peptides
Cyclic GMP
Organelle Biogenesis
Obesity
Muscles
Peroxisome Proliferator-Activated Receptors
High Fat Diet
Blood Vessels
Glucose Intolerance
Guanylate Cyclase
Muscle Cells
Fats
Body Weight
Diet
Peptide Receptors
Brain Natriuretic Peptide
Oxygen Consumption
Energy Metabolism
Insulin Resistance

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Natriuretic peptides/cGMP/cGMP-dependent protein kinase cascades promote muscle mitochondrial biogenesis and prevent obesity. / Miyashita, Kazutoshi; Itoh, Hiroshi; Tsujimoto, Hirokazu; Tamura, Naohisa; Fukunaga, Yasutomo; Sone, Masakatsu; Yamahara, Kenichi; Taura, Daisuke; Inuzuka, Megumi; Sonoyama, Takuhiro; Nakao, Kazuwa.

In: Diabetes, Vol. 58, No. 12, 12.2009, p. 2880-2892.

Research output: Contribution to journalArticle

Miyashita, K, Itoh, H, Tsujimoto, H, Tamura, N, Fukunaga, Y, Sone, M, Yamahara, K, Taura, D, Inuzuka, M, Sonoyama, T & Nakao, K 2009, 'Natriuretic peptides/cGMP/cGMP-dependent protein kinase cascades promote muscle mitochondrial biogenesis and prevent obesity', Diabetes, vol. 58, no. 12, pp. 2880-2892. https://doi.org/10.2337/db09-0393
Miyashita, Kazutoshi ; Itoh, Hiroshi ; Tsujimoto, Hirokazu ; Tamura, Naohisa ; Fukunaga, Yasutomo ; Sone, Masakatsu ; Yamahara, Kenichi ; Taura, Daisuke ; Inuzuka, Megumi ; Sonoyama, Takuhiro ; Nakao, Kazuwa. / Natriuretic peptides/cGMP/cGMP-dependent protein kinase cascades promote muscle mitochondrial biogenesis and prevent obesity. In: Diabetes. 2009 ; Vol. 58, No. 12. pp. 2880-2892.
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AU - Tsujimoto, Hirokazu

AU - Tamura, Naohisa

AU - Fukunaga, Yasutomo

AU - Sone, Masakatsu

AU - Yamahara, Kenichi

AU - Taura, Daisuke

AU - Inuzuka, Megumi

AU - Sonoyama, Takuhiro

AU - Nakao, Kazuwa

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