Natural estrogens induce modulation of microtubules in Chinese hamster V79 cells in culture

Eriko Yokota, A. Susaki, Y. Sato

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Abstract

Natural estrogens and their derivatives comprising 30 compounds in total were tested for their ability to induce microtubule disruption in Chinese hamster V79 cells. The cytoplasmic microtubule networks were examined by the indirect immunofluorescence method using anti-β-tubulin antibody. The effective concentrations of 17β-estradiol (E2-17β) and 17α-estradiol required for the induction of microtubule distribution in 50% of the cells (EC50) in 1 h were 10 and 9 μM for V79 cells, respectively, and 2- methoxyestradiol showed the strongest activity (EC50 2 μM) among the tested compounds including the catechol estrogens 2-hydroxyestradiol, 4- hydroxyestradiol, 2-hydroxyestrone, and 4-hydroxyestrone. The estrone series of estrogens showed relatively low activity for disruption of microtubule networks compared with E2-17β, whereas 3-deoxy-3-methylestradiol showed almost the same EC50 value as E2-17β. There was a correlation between the EC50 values of the compounds and their growth-inhibitory activities. The presence of 1 μM taxol in culture protected against 50 μM E2-17β-induced microtubule disruption. Cycloheximide and actinomycin D had no preventive action on the effect of E2-17β, suggesting that the microtubule-disruptive effect of E2-17β is not associated with newly synthesized proteins and mRNAs. The present results indicate that some natural estrogens cause microtubule disruption in a nongenomic manner.

Original languageEnglish
Pages (from-to)1863-1868
Number of pages6
JournalCancer Research
Volume55
Issue number9
Publication statusPublished - 1995

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Cricetulus
Microtubules
Estrogens
Cell Culture Techniques
Estradiol
Catechol Estrogens
Estrone
Dactinomycin
Cycloheximide
Tubulin
Indirect Fluorescent Antibody Technique
Paclitaxel
Anti-Idiotypic Antibodies
Messenger RNA
Growth
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Natural estrogens induce modulation of microtubules in Chinese hamster V79 cells in culture. / Yokota, Eriko; Susaki, A.; Sato, Y.

In: Cancer Research, Vol. 55, No. 9, 1995, p. 1863-1868.

Research output: Contribution to journalArticle

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