Natural Variation of the Amino-Terminal Glutamine-Rich Domain in Drosophila Argonaute2 Is Not Associated with Developmental Defects

Daniel Hain, Brian R. Bettencourt, Katsutomo Okamura, Tibor Csorba, Wibke Meyer, Zhigang Jin, Jason Biggerstaff, Haruhiko Siomi, Gyorgy Hutvagner, Eric C. Lai, Michael Welte, H. Arno J Müller

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The Drosophila argonaute2 (ago2) gene plays a major role in siRNA mediated RNA silencing pathways. Unlike mammalian Argonaute proteins, the Drosophila protein has an unusual amino-terminal domain made up largely of multiple copies of glutamine-rich repeats (GRRs). We report here that the ago2 locus produces an alternative transcript that encodes a putative short isoform without this amino-terminal domain. Several ago2 mutations previously reported to be null alleles only abolish expression of the long, GRR-containing isoform. Analysis of drop out (dop) mutations had previously suggested that variations in GRR copy number result in defects in RNAi and embryonic development. However, we find that dop mutations genetically complement transcript-null alleles of ago2 and that ago2 alleles with variant GRR copy numbers support normal development. In addition, we show that the assembly of the central RNAi machinery, the RISC (RNA induced silencing complex), is unimpaired in embryos when GRR copy number is altered. In fact, we find that GRR copy number is highly variable in natural D. melanogaster populations as well as in laboratory strains. Finally, while many other insects share an extensive, glutamine-rich Ago2 amino-terminal domain, its primary sequence varies drastically between species. Our data indicate that GRR variation does not modulate an essential function of Ago2 and that the amino-terminal domain of Ago2 is subject to rapid evolution.

Original languageEnglish
Article numbere15264
Pages (from-to)1-14
Number of pages14
JournalPLoS One
Volume5
Issue number12
DOIs
Publication statusPublished - 2010

Fingerprint

Glutamine
glutamine
Drosophila
Defects
RNA Interference
null alleles
Alleles
mutation
Mutation
Protein Isoforms
Argonaute Proteins
RNA-Induced Silencing Complex
Drosophila Proteins
Melanogaster
small interfering RNA
RNA interference
Small Interfering RNA
Machinery
Embryonic Development
Insects

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Hain, D., Bettencourt, B. R., Okamura, K., Csorba, T., Meyer, W., Jin, Z., ... Müller, H. A. J. (2010). Natural Variation of the Amino-Terminal Glutamine-Rich Domain in Drosophila Argonaute2 Is Not Associated with Developmental Defects. PLoS One, 5(12), 1-14. [e15264]. https://doi.org/10.1371/journal.pone.0015264

Natural Variation of the Amino-Terminal Glutamine-Rich Domain in Drosophila Argonaute2 Is Not Associated with Developmental Defects. / Hain, Daniel; Bettencourt, Brian R.; Okamura, Katsutomo; Csorba, Tibor; Meyer, Wibke; Jin, Zhigang; Biggerstaff, Jason; Siomi, Haruhiko; Hutvagner, Gyorgy; Lai, Eric C.; Welte, Michael; Müller, H. Arno J.

In: PLoS One, Vol. 5, No. 12, e15264, 2010, p. 1-14.

Research output: Contribution to journalArticle

Hain, D, Bettencourt, BR, Okamura, K, Csorba, T, Meyer, W, Jin, Z, Biggerstaff, J, Siomi, H, Hutvagner, G, Lai, EC, Welte, M & Müller, HAJ 2010, 'Natural Variation of the Amino-Terminal Glutamine-Rich Domain in Drosophila Argonaute2 Is Not Associated with Developmental Defects', PLoS One, vol. 5, no. 12, e15264, pp. 1-14. https://doi.org/10.1371/journal.pone.0015264
Hain, Daniel ; Bettencourt, Brian R. ; Okamura, Katsutomo ; Csorba, Tibor ; Meyer, Wibke ; Jin, Zhigang ; Biggerstaff, Jason ; Siomi, Haruhiko ; Hutvagner, Gyorgy ; Lai, Eric C. ; Welte, Michael ; Müller, H. Arno J. / Natural Variation of the Amino-Terminal Glutamine-Rich Domain in Drosophila Argonaute2 Is Not Associated with Developmental Defects. In: PLoS One. 2010 ; Vol. 5, No. 12. pp. 1-14.
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