Abstract
Naturally arising CD4+CD25+ regulatory T (T R) cells have been shown to prevent and cure murine T cell-mediated colitis. However, their exact mechanism of controlling colitogenic memory CD4+ T cells in in vivo systems excluding the initial process of naive T cell activation and differentiation has not been examined to date. Using the colitogenic effector memory (TEM) CD4+ cell-mediated colitis model induced by adoptive transfer of colitogenic CD4 +CD44highCD62L- lamina propria (LP) T cells obtained from colitic CD4+CD45RBhigh T cell-transferred mice, we have shown in the present study that CD4+CD25+ TR cells are able not only to suppress the development of colitis, Th1 cytokine production, and the expansion of colitogenic LP CD4+ TEM cells but also to expand these cells by themselves extensively in vivo. An in vitro coculture assay revealed that CD4+CD25+ TR cells proliferated in the presence of IL-2-producing colitogenic LP CD4+ TEM cells at the early time point (48 h after culture), followed by the acquisition of suppressive activity at the late time point (96 h after culture). Collectively, these data suggest the distinct timing of the IL-2-dependent expansion of CD4+CD25+ T R cells and the their suppressive activity on colitogenic LP CD4 + TEM cells.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Gastrointestinal and Liver Physiology |
| Volume | 290 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 May |
| Externally published | Yes |
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Keywords
- Interleukin-2
- Murine colitis model
ASJC Scopus subject areas
- Gastroenterology
- Physiology
Cite this
Naturally arising CD4+CD25+ regulatory T cells suppress the expansion of colitogenic CD4+CD44 highCD62L- effector memory T cells. / Kanai, Takanori; Tanimoto, K.; Nemoto, Y.; Fujii, R.; Makita, S.; Totsuka, T.; Watanabe, M.
In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 290, No. 5, 05.2006.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Naturally arising CD4+CD25+ regulatory T cells suppress the expansion of colitogenic CD4+CD44 highCD62L- effector memory T cells
AU - Kanai, Takanori
AU - Tanimoto, K.
AU - Nemoto, Y.
AU - Fujii, R.
AU - Makita, S.
AU - Totsuka, T.
AU - Watanabe, M.
PY - 2006/5
Y1 - 2006/5
N2 - Naturally arising CD4+CD25+ regulatory T (T R) cells have been shown to prevent and cure murine T cell-mediated colitis. However, their exact mechanism of controlling colitogenic memory CD4+ T cells in in vivo systems excluding the initial process of naive T cell activation and differentiation has not been examined to date. Using the colitogenic effector memory (TEM) CD4+ cell-mediated colitis model induced by adoptive transfer of colitogenic CD4 +CD44highCD62L- lamina propria (LP) T cells obtained from colitic CD4+CD45RBhigh T cell-transferred mice, we have shown in the present study that CD4+CD25+ TR cells are able not only to suppress the development of colitis, Th1 cytokine production, and the expansion of colitogenic LP CD4+ TEM cells but also to expand these cells by themselves extensively in vivo. An in vitro coculture assay revealed that CD4+CD25+ TR cells proliferated in the presence of IL-2-producing colitogenic LP CD4+ TEM cells at the early time point (48 h after culture), followed by the acquisition of suppressive activity at the late time point (96 h after culture). Collectively, these data suggest the distinct timing of the IL-2-dependent expansion of CD4+CD25+ T R cells and the their suppressive activity on colitogenic LP CD4 + TEM cells.
AB - Naturally arising CD4+CD25+ regulatory T (T R) cells have been shown to prevent and cure murine T cell-mediated colitis. However, their exact mechanism of controlling colitogenic memory CD4+ T cells in in vivo systems excluding the initial process of naive T cell activation and differentiation has not been examined to date. Using the colitogenic effector memory (TEM) CD4+ cell-mediated colitis model induced by adoptive transfer of colitogenic CD4 +CD44highCD62L- lamina propria (LP) T cells obtained from colitic CD4+CD45RBhigh T cell-transferred mice, we have shown in the present study that CD4+CD25+ TR cells are able not only to suppress the development of colitis, Th1 cytokine production, and the expansion of colitogenic LP CD4+ TEM cells but also to expand these cells by themselves extensively in vivo. An in vitro coculture assay revealed that CD4+CD25+ TR cells proliferated in the presence of IL-2-producing colitogenic LP CD4+ TEM cells at the early time point (48 h after culture), followed by the acquisition of suppressive activity at the late time point (96 h after culture). Collectively, these data suggest the distinct timing of the IL-2-dependent expansion of CD4+CD25+ T R cells and the their suppressive activity on colitogenic LP CD4 + TEM cells.
KW - Interleukin-2
KW - Murine colitis model
UR - http://www.scopus.com/inward/record.url?scp=33645824349&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645824349&partnerID=8YFLogxK
U2 - 10.1152/ajpgi.00429.2005
DO - 10.1152/ajpgi.00429.2005
M3 - Article
C2 - 16373426
AN - SCOPUS:33645824349
VL - 290
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 5
ER -