Naturally arising CD4+CD25+ regulatory T cells suppress the expansion of colitogenic CD4+CD44 highCD62L- effector memory T cells

T. Kanai, K. Tanimoto, Y. Nemoto, R. Fujii, S. Makita, T. Totsuka, M. Watanabe

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19 Citations (Scopus)


Naturally arising CD4+CD25+ regulatory T (T R) cells have been shown to prevent and cure murine T cell-mediated colitis. However, their exact mechanism of controlling colitogenic memory CD4+ T cells in in vivo systems excluding the initial process of naive T cell activation and differentiation has not been examined to date. Using the colitogenic effector memory (TEM) CD4+ cell-mediated colitis model induced by adoptive transfer of colitogenic CD4 +CD44highCD62L- lamina propria (LP) T cells obtained from colitic CD4+CD45RBhigh T cell-transferred mice, we have shown in the present study that CD4+CD25+ TR cells are able not only to suppress the development of colitis, Th1 cytokine production, and the expansion of colitogenic LP CD4+ TEM cells but also to expand these cells by themselves extensively in vivo. An in vitro coculture assay revealed that CD4+CD25+ TR cells proliferated in the presence of IL-2-producing colitogenic LP CD4+ TEM cells at the early time point (48 h after culture), followed by the acquisition of suppressive activity at the late time point (96 h after culture). Collectively, these data suggest the distinct timing of the IL-2-dependent expansion of CD4+CD25+ T R cells and the their suppressive activity on colitogenic LP CD4 + TEM cells.

Original languageEnglish
Pages (from-to)G1051-G1058
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number5
Publication statusPublished - 2006 May
Externally publishedYes


  • Interleukin-2
  • Murine colitis model

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)


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