Nature of atrial natriuretic polypeptide in rat brain

Shozo Shiono, Kazuwa Nakao, Narito Morii, Takayuki Yamada, Hiroshi Itoh, Makoto Sakamoto, Akira Sugawara, Yoshihiko Saito, Goro Katsuura, Hiroo Imura

Research output: Contribution to journalArticle

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Abstract

Using reverse phase high performance liquid chromatography (RP-HPLC) coupled with two radioimmunoassays for atrial natriuretic polypeptide (ANP) with different specificities, we investigated the nature of α-rat ANP-like immunoreactivity (α-rANP-LI) with a low molecular weight in the rat brain. Two major peaks with α-rANP-LI in the extract from rat whole brains were eluted in the vicinity of the elution position of synthetic α-rANP, a 28-amino acid polypeptide. These two components co-migrated with synthetic α-rANP(4-28) and α-rANP(5-28), respectively. The peak corresponding to α-rANP(4-28) was the highest, and only a little α-rANP-LI was detected at the elution position of α-rANP. An identical profile in RP-HPLC was also observed in the extract from the rat hypothalamus. These results indicate that the major components of α-rANP-LI with a low molecular weight in the rat brain are α-rANP(4-28) and α-rANP(5-28).

Original languageEnglish
Pages (from-to)728-734
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume135
Issue number3
DOIs
Publication statusPublished - 1986 Mar 28
Externally publishedYes

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Rats
Brain
Peptides
High performance liquid chromatography
Reverse-Phase Chromatography
Molecular Weight
Molecular weight
High Pressure Liquid Chromatography
Hypothalamus
Radioimmunoassay
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nature of atrial natriuretic polypeptide in rat brain. / Shiono, Shozo; Nakao, Kazuwa; Morii, Narito; Yamada, Takayuki; Itoh, Hiroshi; Sakamoto, Makoto; Sugawara, Akira; Saito, Yoshihiko; Katsuura, Goro; Imura, Hiroo.

In: Biochemical and Biophysical Research Communications, Vol. 135, No. 3, 28.03.1986, p. 728-734.

Research output: Contribution to journalArticle

Shiono, S, Nakao, K, Morii, N, Yamada, T, Itoh, H, Sakamoto, M, Sugawara, A, Saito, Y, Katsuura, G & Imura, H 1986, 'Nature of atrial natriuretic polypeptide in rat brain', Biochemical and Biophysical Research Communications, vol. 135, no. 3, pp. 728-734. https://doi.org/10.1016/0006-291X(86)90989-7
Shiono, Shozo ; Nakao, Kazuwa ; Morii, Narito ; Yamada, Takayuki ; Itoh, Hiroshi ; Sakamoto, Makoto ; Sugawara, Akira ; Saito, Yoshihiko ; Katsuura, Goro ; Imura, Hiroo. / Nature of atrial natriuretic polypeptide in rat brain. In: Biochemical and Biophysical Research Communications. 1986 ; Vol. 135, No. 3. pp. 728-734.
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