Nature of atrial natriuretic polypeptide in rat brain

Shozo Shiono, Kazuwa Nakao, Narito Morii, Takayuki Yamada, Hiroshi Itoh, Makoto Sakamoto, Akira Sugawara, Yoshihiko Saito, Goro Katsuura, Hiroo Imura

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92 Citations (Scopus)


Using reverse phase high performance liquid chromatography (RP-HPLC) coupled with two radioimmunoassays for atrial natriuretic polypeptide (ANP) with different specificities, we investigated the nature of α-rat ANP-like immunoreactivity (α-rANP-LI) with a low molecular weight in the rat brain. Two major peaks with α-rANP-LI in the extract from rat whole brains were eluted in the vicinity of the elution position of synthetic α-rANP, a 28-amino acid polypeptide. These two components co-migrated with synthetic α-rANP(4-28) and α-rANP(5-28), respectively. The peak corresponding to α-rANP(4-28) was the highest, and only a little α-rANP-LI was detected at the elution position of α-rANP. An identical profile in RP-HPLC was also observed in the extract from the rat hypothalamus. These results indicate that the major components of α-rANP-LI with a low molecular weight in the rat brain are α-rANP(4-28) and α-rANP(5-28).

Original languageEnglish
Pages (from-to)728-734
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 1986 Mar 28
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Shiono, S., Nakao, K., Morii, N., Yamada, T., Itoh, H., Sakamoto, M., Sugawara, A., Saito, Y., Katsuura, G., & Imura, H. (1986). Nature of atrial natriuretic polypeptide in rat brain. Biochemical and Biophysical Research Communications, 135(3), 728-734.