NE-dlg, a mammalian homolog of Drosophila dlg tumor suppressor, induces growth suppression and impairment of cell adhesion: Possible involvement of down-regulation of β-catenin by NE-dlg expression

Norihisa Hanada, Keishi Makino, Hisashi Koga, Tetsuro Morisaki, Hiroaki Kuwahara, Norio Masuko, Yoichi Tabira, Takehisa Hiraoka, Nobuo Kitamura, Akira Kikuchi, Hideyuki Saya

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Membrane-associated guanylate kinases (MAGUK's) are known to function as scaffolds for forming multiprotein complexes at the synaptic junctions of neuronal cells and at sites of epithelial cell-cell contact. In Drosophila, mutations of the lethal (I)-discs large (dig) gene, which encodes a MAGUK protein, leads to post-synaptic structure defects in neuronal cells and neoplastic overgrowth of epithelial cells. We previously showed that NE-dig (neuronal and endocrine dig), a human homolog of the dig, plays a crucial role in formation of synaptic structure in human neuronal cells. Here we demonstrate that NE-dig, similar to Drosophila dig, is involved in regulation of cell cycle progression and adhesive ability of non-neuronal cells. Overexpression of NE-dlg in proliferating cells including various cancer cell lines induced growth suppression and impairment of cell adhesive ability. Furthermore, NE-dlg overexpression caused the down-regulation of β-catenin in cancer cells regardless of mutations in the APC (adenomatous polyposis coil) gene. The PDZ domains of NE-dlg were found to be essential for the growth suppression, loss of adhesive property and down-regulation of β- catenin. We propose that NE-dlg regulates the cell growth and adhesive ability by controlling the level of β-catenin through an APC-independent pathway. Inactivation of NE-dlg may therefore contribute to development and/or progression of human neoplasms. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)480-488
Number of pages9
JournalInternational Journal of Cancer
Volume86
Issue number4
DOIs
Publication statusPublished - 2000
Externally publishedYes

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Catenins
Cell Adhesion
Drosophila
Down-Regulation
Growth
Adhesives
Neoplasms
Guanylate Kinases
Epithelial Cells
PDZ Domains
Multiprotein Complexes
Mutation
Intercellular Junctions
Genes
Cell Cycle
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

NE-dlg, a mammalian homolog of Drosophila dlg tumor suppressor, induces growth suppression and impairment of cell adhesion : Possible involvement of down-regulation of β-catenin by NE-dlg expression. / Hanada, Norihisa; Makino, Keishi; Koga, Hisashi; Morisaki, Tetsuro; Kuwahara, Hiroaki; Masuko, Norio; Tabira, Yoichi; Hiraoka, Takehisa; Kitamura, Nobuo; Kikuchi, Akira; Saya, Hideyuki.

In: International Journal of Cancer, Vol. 86, No. 4, 2000, p. 480-488.

Research output: Contribution to journalArticle

Hanada, Norihisa ; Makino, Keishi ; Koga, Hisashi ; Morisaki, Tetsuro ; Kuwahara, Hiroaki ; Masuko, Norio ; Tabira, Yoichi ; Hiraoka, Takehisa ; Kitamura, Nobuo ; Kikuchi, Akira ; Saya, Hideyuki. / NE-dlg, a mammalian homolog of Drosophila dlg tumor suppressor, induces growth suppression and impairment of cell adhesion : Possible involvement of down-regulation of β-catenin by NE-dlg expression. In: International Journal of Cancer. 2000 ; Vol. 86, No. 4. pp. 480-488.
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abstract = "Membrane-associated guanylate kinases (MAGUK's) are known to function as scaffolds for forming multiprotein complexes at the synaptic junctions of neuronal cells and at sites of epithelial cell-cell contact. In Drosophila, mutations of the lethal (I)-discs large (dig) gene, which encodes a MAGUK protein, leads to post-synaptic structure defects in neuronal cells and neoplastic overgrowth of epithelial cells. We previously showed that NE-dig (neuronal and endocrine dig), a human homolog of the dig, plays a crucial role in formation of synaptic structure in human neuronal cells. Here we demonstrate that NE-dig, similar to Drosophila dig, is involved in regulation of cell cycle progression and adhesive ability of non-neuronal cells. Overexpression of NE-dlg in proliferating cells including various cancer cell lines induced growth suppression and impairment of cell adhesive ability. Furthermore, NE-dlg overexpression caused the down-regulation of β-catenin in cancer cells regardless of mutations in the APC (adenomatous polyposis coil) gene. The PDZ domains of NE-dlg were found to be essential for the growth suppression, loss of adhesive property and down-regulation of β- catenin. We propose that NE-dlg regulates the cell growth and adhesive ability by controlling the level of β-catenin through an APC-independent pathway. Inactivation of NE-dlg may therefore contribute to development and/or progression of human neoplasms. (C) 2000 Wiley-Liss, Inc.",
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T2 - Possible involvement of down-regulation of β-catenin by NE-dlg expression

AU - Hanada, Norihisa

AU - Makino, Keishi

AU - Koga, Hisashi

AU - Morisaki, Tetsuro

AU - Kuwahara, Hiroaki

AU - Masuko, Norio

AU - Tabira, Yoichi

AU - Hiraoka, Takehisa

AU - Kitamura, Nobuo

AU - Kikuchi, Akira

AU - Saya, Hideyuki

PY - 2000

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